中耳胆脂瘤中Rock 1和Rock 2的表达研究

Study on the Expression of Rock 1 and Rock 2 in Middle Ear Cholesteatoma

宋少鹏;马秀岚;

1:中国医科大学附属盛京医院耳科

摘要

目的探讨Rho相关卷曲螺旋蛋白激酶1(Rho-associated coiled coil-containing protein kinase 1,Rock1)和Rho相关卷曲螺旋蛋白激酶2(Rock2)在胆脂瘤发生过程中的作用。方法采用免疫组织化学方法检测27例中耳胆脂瘤标本(病例组)和20例外耳道皮肤组织标本(对照组)中Rock1和Rock 2的表达。结果 Rock1蛋白阳性表达定位于细胞质,其在中耳胆脂瘤上皮中阳性表达率为77.78%(21/27),高于对照组外耳道皮肤的25.0%(5/20),差异有统计学意义(P<0.05);Rock 2蛋白阳性表达主要定位于细胞质,其在中耳胆脂瘤上皮的阳性表达率为25.93%(7/27),低于对照组外耳道皮肤的90.0%(18/20),差异有统计学意义(P<0.05);在中耳胆脂瘤上皮组织中,Rock 1和Rock 2蛋白的表达呈负相关(r=-0.497,P=0.008<0.05)。结论中耳胆脂瘤上皮中Rock1高表达和Rock2的低表达可能在中耳胆脂瘤角质细胞的分化过程中起重要作用。

关键词

胆脂瘤;Rho相关卷曲螺旋蛋白激酶1;Rho相关卷曲螺旋蛋白激酶2;细胞分化

基金项目(Foundation):

辽宁省自然科学基金项目(2014021019)

作者

宋少鹏;马秀岚;

参考文献

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胆脂瘤 Rho相关卷曲螺旋蛋白激酶1 Rho相关卷曲螺旋蛋白激酶2 细胞分化

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宋少鹏马秀岚