鼓室内接种耐甲氧西林金黄色葡萄球菌构建大鼠鼓室硬化模型

Intratympanic Inoculation of Methicillin-resistant Staphylococcus Aureus for Establishment of an Experimental Tympanosclerosis Model in Rats

黄玉;刘磊;刘爱国;

1:华中科技大学同济医学院附属同济医院耳鼻咽喉-头颈外科

2:湖北省京山市人民医院

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摘要

目的探讨鼓室内接种耐甲氧西林金黄色葡萄球菌(methicillin-resistant staphylococcus aureus,MRSA)建立大鼠鼓室硬化模型的可行性。方法 34只SD大鼠被随机分为实验组(n=17)和对照组(n=17),实验组大鼠左耳鼓室内接种MRSA,右耳不予处理,对照组大鼠双耳都不予处理。于造模后第1、2、4、6周观察两组大鼠双耳鼓膜变化,并于造模后第6周取对照组大鼠左耳听泡及实验组大鼠双耳听泡行石蜡切片,HE染色后观察两组大鼠鼓膜及鼓室粘膜的形态学变化。结果造模后1周,实验组大鼠均存活,无鼓室硬化形成;造模后2周,实验组大鼠因肺部感染死亡1只,余下大鼠中有4只左耳发生鼓室硬化;造模后4周,实验组大鼠因肺部感染死亡2只,因麻醉过量死亡1只,余下大鼠中有10耳发生鼓室硬化,左侧7耳,右侧3耳;造模后6周,实验组大鼠因肺部感染死亡2只,余下大鼠中有12耳发生鼓室硬化(12/22,54.5%),左侧7耳,右侧5耳;粘膜增厚、炎性细胞浸润、纤维组织增生以及间质组织增厚等形态学改变不仅见于实验组接种细菌耳,亦可见于对侧耳。对照组大鼠因麻醉过量死亡2只,余15只存活至6周末,各大鼠双耳均未出现任何异常。结论大鼠鼓室内接种MRSA是一种新的构建鼓室硬化模型的方法,可成功建立大鼠鼓室硬化模型。

关键词

鼓室硬化;耐甲氧西林金黄色葡萄球菌;大鼠

基金项目(Foundation):

作者

黄玉;刘磊;刘爱国;

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鼓室硬化耐甲氧西林金黄色葡萄球菌大鼠

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黄玉刘磊刘爱国