siRNA抑制GRP75表达对叔丁基双氧水诱导HEI-OC1毛细胞株凋亡的影响
Effect of Inhibition GRP75 by siRNA on Apoptosis of HEI-OC1 Cells Induced by t-BHP
陈佩珊;刘淑仪;张敏;王军义;
1:广东药科大学公共卫生学院
摘要关键词基金项目(Foundation):作者参考文献1 Op de Beeck K,Schacht J,Van Camp G.Apoptosis in acquired and genetic hearing impairment:the programmed death of the hair cell[J].Hear Res,2011,281:18.
2 Kamogashira T,Fujimoto C,Yamasoba T.Reactive oxygen species,apoptosis,and mitochondrial dysfunction in hearing loss[J].Biomed Res Int,2015,2015:617207.
3 Kurabi A,Keithley EM,Housley GD,et al.Cellular mechanisms of noise-induced hearing loss[J].Hear Res.2017,349:129.
4 Londono C,Osorio C,Gama V,et al.Mortalin,apoptosis,and neurodegeneration[J].Biomolecules,2012,2:143.
5 Yang H,Zhou X,Liu X,et al.Mitochondrial dysfunction induced by knockdown of mortalin is rescued by Parkin[J].Biochem Biophys Res Commun,2011,410:114.
6 张菲玲,夏云,姚裕恒,等.热休克蛋白75/78在叔丁基双氧水诱导HEI-OC1毛细胞凋亡中的作用[J].听力学及言语疾病杂志,2018,26:419.
7 Park C,Thein P,Kalinec G,et al.Hei-ocl cells as a model for investigating prestin function[J].Hear Res,2016,335:9.
8 Wang Z,Liu YM,Han N,et al.Profiles of oxidative stress-related microrna and mrna expression in auditory cells[J].Brain Research,2010,1346:14.
9 Fire A,Xu S,Montgomery MK,et al.Potent and specific genetic interference by double-stranded RNA in caenorhabditis elegans[J].Nature,1998,391:806.
10 Joshi BH,Pachchigar KP.siRNA:novel therapeutics from functional genomics[J].Biotechnol Genet Eng Rev,2014,30:1.
11 Deocaris CC,Kaul SC,Wadhwa R.From proliferative to neurological role of an hsp70 stress chaperone,mortalin[J].Biogerontology,2008,9:391.
12 Zhang X,Beuron F,Freemont PS.Machinery of protein folding and unfolding[J].Curr Opin Struct Biol,2002,12:231.
13 Choudhary GS,Al-Harbi S,Almasan A.Caspase-3 activation is a critical determinant of genotoxic stress-induced apoptosis[J].Methods Mol Biol,2015,1219:1.
14 Starenki D,Hong SK,Lloyd RV,et al.Mortalin(GRP75/HSPA9) upregulation promotes survival and proliferation of medullary thyroidcarcinoma cells[J].Oncogene,2015,34:4624.
15 Park SH,Baek KH,Shin I,et al.Subcellular Hsp70 inhibitors promote cancer cell death via different mechanisms[J].Cell Chem Biol,2018,25:1242.
16 Pridgeon JW,Olzmann JA,Chin LS,et al.PINK1 protects against oxidative stress by phosphorylating mitochondrial chaperone TRAP1[J].PLos Biol,2007,5:e172.
17 Yang L,Guo W,Zhang Q,et al.Crosstalk between Raf/MEK/ERK and PI3K/AKT in suppression of Bax conformational change by Grp75 under glucose deprivation conditions[J].J Mol Biol,2011,414:654.