靶向抑制TANK结合激酶1表达对舌及下咽鳞癌细胞增殖及凋亡的影响

Effects of Targeted Inhibition of TANK Binding Kinase 1 Expression on Biological Behavior of Squamous Cells Carcinoma of Tongue and Pharynx

王亚平;邱雁君;李玲香;崔晓波;张莉;胡文良;贺利平;

1:内蒙古医科大学附属医院耳鼻咽喉科

2:内蒙古医科大学附属医院重症医学科

摘要
目的分析靶向抑制头颈部鳞癌细胞中TANK结合酶1(TANK binding,kinase 1,TBK1)表达后细胞增殖与凋亡水平改变。方法采用转染TBK1siRNA靶向抑制人下咽鳞癌细胞FaDu、人舌鳞癌细胞Cal-27中TBK1的表达,用CCK-8、流式细胞术、Western Blot等方法检测FaDu及Cal-27中靶向抑制TBK1表达前后细胞的增殖及凋亡水平;克隆形成实验及细胞增殖实验(CCK-8法)检测梯度浓度氨来呫诺处理后FaDu及Cal-27细胞增殖改变。结果Western Blot结果显示,转染后FaDu及Cal-27细胞中TBK1蛋白表达显著抑制;细胞增殖实验结果显示,靶向抑制TBK1蛋白表达后,FaDu及Cal-27的增殖显著抑制;流式细胞术检测显示FaDu及Cal-27细胞凋亡显著增加;克隆形成及细胞增殖实验结果表明FaDu及Cal-27细胞的增殖随着氨来呫诺浓度增加呈现显著下降(P<0.05)。结论靶向抑制TBK1蛋白表达后,舌及下咽鳞癌细胞的增殖能力明显降低,细胞凋亡显著增加;TBK1抑制剂氨来呫诺对于高表达TBK1的舌及下咽鳞状细胞癌的增殖具有抑制作用。
关键词
舌;下咽;鳞状细胞癌;TANK结合激酶1;氨来呫诺
基金项目(Foundation):
内蒙古医科大学青年创新基金(YKD2015QNCX030);内蒙古医科大学科技百万工程项目(YKD2017JBW(LH)012);; 内蒙古自然科学基金(2017MS(LH)0832)
作者
王亚平;邱雁君;李玲香;崔晓波;张莉;胡文良;贺利平;
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