电针耳穴通过SIRT1/PGC-1α途径延缓D-半乳糖致衰老豚鼠听皮层老化
Effect of Electroacupuncture at Tinggong and Yifeng on D-galactose-induced Aging of Auditory Cortex through SIRT1/PGC-1α Pathway in Guinea Pigs
帅常娟;刘淑云;姚宇;杨烨;殷泽登;
1:西南医科大学附属医院耳鼻咽喉头颈外科
2:西南医科大学基础医学院生物化学教研室





1 Mikhed Y,Daiber A,Steven S,et al.Mitochondrial oxidative stress mitochondrial DNA damage and their role in agerelated vascular dysfunction[J].Int J MolSci,2015,16:15918.
2 Venkataraman K,Khurana S,Tai TC,et al.Oxidative stress in aging-matters of the heart and mind[J].Int J MolSci,2013,14:17897.
3 Sánchez-Rodríguez C,Martín-Sanz E,Cuadrado E,et al.Protective effect of polyphenols on presbycusis via oxidative/nitrosative stress suppression in rats[J].Exp Gerontol,2016,83:31.
4 Cheng Y,Takeuchi H,Sonobe Y,et al.Sirtuin 1attenuates oxidative stress via upregulation of superoxide dismutase 2and catalase in astrocytes[J].Neuroimmunol,2014,269:38.
5 Lee YH,Chen HY,Su LJ,et al.Sirtuin 1(SIRT1)deacetylase activity and NAD+/NADH ratio are imperative for capsaicin-mediated programmed cell death[J].Agric Food Chem,2015,63:7361.
6 Liu H,Sheng M,Liu Y,et al.Expression of SIRT1and oxidative stress in diabetic dry eye[J].Int J Clin Exp Pathol,2015,8:7644.
7 Tan M,Tang C,Zhang Y,et al.SIRT1/PGC-1αsignaling protects hepa-tocytes against mitochondrial oxidative stress induced by bile acids[J].Free Radic Res,2015,49:935.
8 Basu S.A complex interplay between PGC-1co-activators and mTORC1regulates hematopoietic recovery following 5-fluorouracil treatment[J].Stem Cell Res,2014,12:178.
9 李君梅,殷泽登,谢仕津,等.电针耳穴对年龄相关性聋豚鼠耳蜗核、下丘和听皮层超氧化物歧化酶活性的影响[J].中国老年学杂志,2013,33:5608.
10 Li FH,Yu HT,Xiao L,et al.Response of BAX,Bcl-2Proteins,and SIRT1/PGC-1αmRNA expression to 8-week treadmill running in the aging rat skeletal muscle[J].AdvExp Med Biol,2016,923:283.
11 Kou X,Li J,Liu X,et al.Ampelopsin attenuates the atrophy of skeletal muscle from D-gal-induced aging rats through activating AMPK/SIRT1/PGC-1αsignaling cascade[J].Biomed Pharmacother,2017,90:311.
12 Tian G,Sawashita J,Kubo H,et al.Ubiquinol-10supplementation activates mitochondria functions to decelerate senescence insenescence-accelerated mice[J].Antioxid Redox Signal,2014,20:2606.
13 赵铭辉,李胜,谯凤英,等.国内针灸治疗突发性聋临床随机对照试验文献分析[J].中国中西医结合耳鼻咽喉科杂志,2010,18:244.
14 Albuquerque AA,Rossato M,Oliveira JA,et al.Understanding the anatomy of ears from guinea pigs and rats and its use in basic otologic research[J].Braz J Otorhinolaryngol,2009,75:43.
15 谢仕津,殷泽登,李君梅,等.电针耳穴对D-半乳糖致豚鼠年龄相关性聋听觉中枢丙二醛表达的影响[J].听力学及言语疾病杂志,2012,20:254.
16 Kong WJ,Yin ZD,Fan GR,et al.Time course of neuronal and synaptic plasticity in dorsal cochlear nucleus of guinea pig following chronic kanamycin-induced deafness[J].Brain Res,2010,1328:118.
17 Schofield BR,Coomes DL.Auditory cortical projections to the cochlear nucleus in guinea pigs[J].Hear Res,2005,199:89.
18 Khan-Malek R,Wang Y.Statistical analysis of quantitative RT-PCR results[J].Methods Mol Biol,2017,1641:281.
19 秦红兵,杨朝晔,范忆江,等.D-半乳糖诱导衰老小鼠模型的建立与评价[J].中国组织工程研究与临床康复,2009,13:1275.
20 刘淑云,邓力强,杨烨,等.电针耳穴对年龄相关性听力损失豚鼠听觉中枢β-catenin表达的影响[J].听力学及言语疾病杂志,2016,24:593.
21 Wang SJ,Zhao XH,Chen W,et al.Sirtuin 1activation enhances the PGC-1α/mitochondrial antioxidant system pathway in status epilepticus[J].Mol Med Rep,2015,11:521.
22 Bu XS,Wu D,Lu XM,et al.Role of SIRT1/PGC-1αin mitochondrial oxidative stress in autistic spectrum disorder[J].Neuropsychiatr Dis Treat,2017,13:1633.
23 Di Emidio G,Falone,Vitti M,et al.SIRT1signalling protects mouse oocytes against oxidative stress and is deregulated during aging[J].Hum Reprod,2014,29:2006.
24 Xue T,Wei L,Zha DJ,et al.miR-29boverexpression induces cochlear hair cell apoptosis through the regulation of SIRT1/PGC-1αsignaling:implications for age-related hearing loss[J].Int J Mol Med,2016,38:1387.
25 Frisina RD,Ding B,Zhu X,et al.Age-related hearing loss:prevention of threshold declines,cell loss andapoptosis in spiral ganglion neurons[J].Aging(Albany NY),2016,8:2081.
26 康颂建,史献君,史永芝,等.电针对庆大霉素耳中毒的防治作用及其机制的研究[J].中国应用生理学杂志,2001,17:285.
27 张晓哲.电针针刺治疗耳鸣临床疗效对比研究[J].中国针灸,2002,22:91.
28 涂厚义,陈茜,刘淑云,等.电针听宫和翳风穴对豚鼠听皮层糖代谢及IGF-1表达的影响[J].听力学及言语疾病杂志,2015,23:280.
29 Dong W,Quo W,Wang F,et al.Electroacupuncture upregulates SIRT1-dependent PGC-1αexpression in SAMP8mice[J].Med Sci Monit,2015,21:3356.
30 Jung YS,Lee SW,Park JH,et al.Electroacupuncture preconditioning reduces ROS generation with NOX4down-regulation and ameliorates blood-brain barrier disruption after ischemic stroke[J].J Biomed Sci,2016,23:32.