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不同年龄段感音神经性聋患者谷氨酸受体7基因多态性分析

Association between GRM7 Gene Polymorphism and Sensorineural Hearing Loss in Different Ages

王文涛;陈贤明;谢三林;郭文玲;

1:南京军区福州总医院耳鼻咽喉头颈外科

2:福建医科大学福总临床医学院

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摘要

目的研究不同年龄段感音神经性聋患者代谢型谷氨酸受体7(GRM7)基因rs11928865、rs11920109位点单核苷酸多态性(SNP)及其与年龄的相关性。方法研究对象为906例感音神经性聋患者和755例健康体检者,按年龄段分为试验组(感音神经性聋A组和B组)和对照组(健康体检者C组和D组),A组467例(3050岁),平均听阈50.91±19.34dB HL,B组439例(5150岁),平均听阈50.91±19.34dB HL,B组439例(5170岁),平均听阈53.53±18.44dB HL,C组278例(3070岁),平均听阈53.53±18.44dB HL,C组278例(3050岁),平均听阈9.22±6.39dB HL,D组477例(5150岁),平均听阈9.22±6.39dB HL,D组477例(5170岁),平均听阈11.33±6.25dB HL。各组分别采集外周血抽提DNA,应用Sequenom MassARRAY方法进行SNP位点分析。对试验组与对照组间的GRM7基因rs11928865、rs11920109位点基因型及等位基因频率进行统计学分析。结果5170岁),平均听阈11.33±6.25dB HL。各组分别采集外周血抽提DNA,应用Sequenom MassARRAY方法进行SNP位点分析。对试验组与对照组间的GRM7基因rs11928865、rs11920109位点基因型及等位基因频率进行统计学分析。结果5170岁年龄段感音神经性聋组与对照组间rs11928865位点基因型分布差异有统计学意义(P=0.018),3070岁年龄段感音神经性聋组与对照组间rs11928865位点基因型分布差异有统计学意义(P=0.018),3050岁年龄段感音神经性聋组与对照组间差异无统计学意义(P>0.05);rs11920109位点基因型在3050岁年龄段感音神经性聋组与对照组间差异无统计学意义(P>0.05);rs11920109位点基因型在3050岁及5150岁及5170岁年龄段感音神经性聋组与对照组间差异均无统计学意义(P>0.05)。结论 GRM7基因rs11928865位点多态性与5170岁年龄段感音神经性聋组与对照组间差异均无统计学意义(P>0.05)。结论 GRM7基因rs11928865位点多态性与5170岁年龄段感音神经性聋易感性相关,但与3070岁年龄段感音神经性聋易感性相关,但与3050岁年龄段感音神经性聋易感性无相关性。

关键词

感音神经性聋;代谢型谷氨酸受体7;单核苷酸多态性

基金项目(Foundation):

福建省自然科学基金项目(2015J01405)资助

作者

王文涛;陈贤明;谢三林;郭文玲;

参考文献

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