热休克蛋白75/78在叔丁基双氧水诱导HEI-OC1毛细胞株凋亡中的作用

Effect of GRP75/78 on Apoptosis of HEI-OC1 Cells Induced by t-BHP

张菲玲;夏云;姚裕恒;王军义;

1:广东药科大学公共卫生学院

摘要

目的分析氧化应激损伤的HEI-OC1毛细胞中热休克蛋白75/78(GRP75/78)的表达水平,探讨GRP75、GRP78在耳蜗毛细胞凋亡过程中的可能作用。方法用不同浓度(20、40、60μM)叔丁基过氧化氢(tBHP)染毒构建HEI-OC1毛细胞株凋亡模型,对照组不加t-BHP,培养24h后用Western blot检测各组毛细胞凋亡标志物Caspase-3、Active-Caspase-3表达水平,实时荧光定量PCR检测GRP75、GRP78mRNA表达水平。结果与对照组相比,各染毒组Caspase-3和Active-Caspase-3蛋白表达水平显著增高(F=1 196.741,P<0.001和F=184.131,P<0.001),40μM和60μM染毒组Caspase-3和Active-Caspase-3蛋白表达水平显著高于20μM染毒组(P<0.01),但40μM和60μM染毒组之间差异无统计学意义(P>0.05)。与对照组相比较,各染毒组GRP75和GRP78mRNA表达水平显著增高(F=228.065,P<0.001和F=1 298.057,P<0.001),40μM和60μM染毒组GRP75和GRP78mRNA表达水平显著高于20μM染毒组(P<0.01),但40μM和60μM染毒组之间差异无统计学意义(P>0.05)。结论 GRP75和GRP78在氧化应激损伤的耳蜗毛细胞中有抗凋亡的生物学作用。

关键词

HEI-OC1细胞;凋亡;热休克蛋白75;热休克蛋白78

基金项目(Foundation):

广东省科技计划项目(2014A0202126);; 广东省2017年大学生创新创业训练计划项目(201710573034)

作者

张菲玲;夏云;姚裕恒;王军义;

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HEI-OC1细胞凋亡热休克蛋白75 热休克蛋白78

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张菲玲夏云姚裕恒王军义