水合氯醛对豚鼠听性脑干反应的影响

Chloral Hydrate Effect on Audrtory Brainstem Response in Guinea Pig

程馨;杨驹;吴楷文;李国庆;赵立东;马秀岚;

1:中国医科大学附属盛京医院

2:中国人民解放军总医院 解放军耳鼻喉研究所

摘要
目的探讨水合氯醛麻醉对成年豚鼠听性脑干反应检测的影响。方法分别在清醒状态(waking state,W)及水合氯醛(chloral hydrate,CH)麻醉状态下对20只健康成年雄性白色豚鼠进行短声(click)诱发的听性脑干反应(auditory brainstem response,ABR)检测,以引出率最高的波来判断反应阈值,比较两种状态下90dB peSPL刺激声强下ABR各波的潜伏期、波间期及不同强度下的Ⅱ、Ⅲ、Ⅳ波振幅及引出率。结果 20只豚鼠水合氯醛麻醉状态与清醒状态下ABR反应阈分别为25.50±2.76、28.5±3.66dB peSPL,差异无统计学意义(P>0.05)。水合氯醛麻醉状态下ABR各波潜伏期较清醒状态均延长,Ⅲ、Ⅳ、Ⅴ波差异有统计学意义(P<0.05),而Ⅰ、Ⅱ波差异无统计学意义(P>0.05);水合氯醛麻醉状态下Ⅰ-Ⅴ、Ⅲ-Ⅳ、Ⅳ-Ⅴ波间期较清醒状态下延长(P<0.05),而Ⅰ-Ⅱ、Ⅱ-Ⅲ波间期差异无统计学意义(P>0.05);水合氯醛麻醉状态和清醒状态均显示波Ⅱ振幅及引出率最高;水合氯醛麻醉状态波Ⅱ、Ⅲ振幅在高强度刺激声时较清醒状态高,而波Ⅳ振幅较清醒状态低(P<0.05)。结论水合氯醛麻醉及清醒状态下豚鼠ABR反应阈无明显差异,但水合氯醛麻醉会使豚鼠ABR的波Ⅲ、Ⅳ、Ⅴ潜伏期明显延长,并对振幅产生影响;豚鼠ABR波Ⅱ出现率最高,可以Ⅱ波判断反应阈。
关键词
麻醉药;水合氯醛;听性脑干反应;豚鼠
基金项目(Foundation):
国家重大科学研究计划项目(2014CB943001);; 国家自然基金重点国际合作项目(81120108009);; 国家自然科学基金重点项目(81530032)、面上项目(81271082);; 全军“十二五”重点项目(BWS11J026)联合资助
作者
程馨;杨驹;吴楷文;李国庆;赵立东;马秀岚;
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