体外诱导人脐带间充质干细胞向内耳毛细胞样细胞分化的研究
Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Hair-cell Like Cells in Vitro
张婷;叶放蕾;关方霞;马珊珊;朱晓丹;赵堃;王乐;李红敏;郝少娟;
1:郑州大学第一附属医院耳鼻喉科
2:郑州大学生命科学学院





1 Matsui JI,Parker MA,Ryals BM,et al.Regeneration and replacement in the vertebrate inner ear[J].Drug Discov Today,2005,10:1307.
2 Li H,Liu H,Heller S.Pluripotent stem cells from the adult mouse inner ear[J].Nat Med,2003,9:1293.
3 Oshima K.Therapeutic approaches using in vitro induction system of hair cells from ES/iPS cells[J].Folia Pharmacol Jpn,2013,141:195.
4 Chen S,Zhang W,Wang JM,et al.Differentiation of isolated human umbilical cord mesenchymal stem cells into neural stem cells[J].Int J Ophthalmol.2016,9:41.
5 Raphael Y.Cochlear pathology,sensory cell death and regeneration[J].Br Med Bull,2002,63:25.
6 Hudspeth AJ.How hearing happens[J].Neuron,1997,19:947.
7 Oshima K,Shin K,Diensthuber M,et al.Mechanosensitive hair cell-like cells from embryonic and induced pluripotent stem cells[J].Cell,2010,141:704.
8 Sarugaser R,Lickorish D,Baksh D,et al.Human umbilical cord perivascular(HUCPV)cells:a source of mesenchymal progenitors[J].Stem Cells,2005,23:220.
9 Ciccolini F,Svendsen CN.Fibroblast growth factor 2(FGF-2)promotes acquisition of epidermal growth factor(EGF)responsiveness in mouse striatal precursor cells:identification of neural precursors responding to both EGF and FGF-2[J].JNeurosci,1998,18:7869.
10 Brewer GJ.Regeneration and proliferation of embryonic and adult rat hippocampal neurons in culture[J].Exp Neurol,1999,159:237.
11 Kondo T,Johnson SA,Yoder MC,et al.Sonic hedgehog and retinoic acid synergistically promote sensory fate specification from bone marrow-derived pluripotent stem cells[J].Proc Natl Acad Sci,2005,102:4789.
12 Kelley MW,Xu XM,Wagner MA,et al.The developing organ of Corti contains retinoic acid and forms supernumerary hair cells in response to exogenous retinoic acid in culture[J].Development,1993,119:1041.
13 Cai T,Seymour ML,Zhang H,et al.Conditional deletion of Atoh1reveals distinct critical periods for survival and function of hair cells in the organ of Corti[J].J Neurosci,2013,33:10110.
14 Monroe JD,Manning DP,Uribe PM,et al.Hearing sensitivity differs between zebrafish lines used in auditory research[J].Hear Res,2016,341:220.
15管利娜,陈燕红,朱恒清,等.小鼠诱导性多潜能干细胞向内耳毛细胞和螺旋神经节细胞分化的体外研究[J].中华耳鼻咽喉头颈外科杂志,2014,49:680.