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两种鼓室硬化造模方法的比较

Comparison of two Methods for the Modeling of Tympanosclerosis in Rat

张艳飞;郑烨贤;王素洁;彭丽艳;何超;刘爱国;

1:华中科技大学同济医学院附属同济医院耳鼻咽喉-头颈外科

2:武汉市中心医院耳鼻咽喉-头颈外科

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摘要

目的应用鼓膜切开法和鼓室接种细菌法构建鼓室硬化动物模型,观察两种方法造模后其鼓膜和鼓室粘膜的组织形态学变化。方法 40只SD大鼠分为4组,每组10只:A1组双耳鼓膜不作处理,作为正常对照;A2组双耳鼓膜紧张部后下象限作2mm的切口;B1组双侧鼓室不作处理,作为正常对照;B2组双侧鼓室接种1×108 CFU/ml肺炎链球菌0.1ml。分别于鼓膜切开后第2周及鼓室接种肺炎链球菌后第1、2、4、6、8周时观察各组大鼠鼓膜情况,比较鼓室硬化发生率;分别于造模后第2周和第8周取材行听泡连续切片HE染色,观察鼓膜及鼓室粘膜的组织形态学变化。结果 A2组14耳发生鼓膜钙化(14/20,70%);B2组死亡一只,6耳发生鼓膜钙化(6/18,33.33%),两组的鼓膜及鼓室粘膜均可观察到炎症细胞浸润及纤维组织增生,但B2组的改变更显著。两对照组(A1和B1组)既未发生鼓膜钙化,也未出现组织形态学改变。结论两种鼓室硬化造模方法均切实可行,但鼓膜切开法操作更简单,成模周期更短,成模率更高。

关键词

鼓室硬化;大鼠;鼓膜切开;肺炎链球菌

基金项目(Foundation):

作者

张艳飞;郑烨贤;王素洁;彭丽艳;何超;刘爱国;

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