噪声对豚鼠耳蜗基底膜乙酰化组蛋白H2B表达的影响

Noise-Induced Acetyl-histone H2B Expression Alteration in Cochlear Basilar Membrane of Guinea Pigs

杨登化;龚树生;陈丽艳;谢静;

1:首都医科大学附属复兴医院耳鼻喉头颈外科

2:首都医科大学附属北京友谊医院耳鼻喉头颈外科

摘要
目的观察噪声性聋豚鼠耳蜗基底膜毛细胞乙酰化组蛋白H2B表达水平的变化,探讨组蛋白乙酰化与噪声性听力损失的相关性。方法成年雄性白色红目豚鼠60只随机分为噪声组和对照组,两组豚鼠分别进行听性脑干反应(ABR)检测后,噪声组给予高强度窄带噪声(122dB SPL,3小时)暴露,对照组不予任何处理;噪声组于噪声暴露后1小时、3天、7天、14天和21天分别行ABR检测;并以免疫荧光染色和Western blot方法检测两组豚鼠耳蜗基底膜听觉细胞中乙酰化组蛋白H2B的表达水平。结果噪声暴露后1小时、3天、7天、14天、21天噪声组豚鼠ABR各频率反应阈均较噪声暴露前及对照组显著增高,且随时间的延长听力逐渐恢复,14天时趋于稳定,达到永久性阈移。免疫荧光染色显示在噪声组豚鼠耳蜗内外毛细胞及Hensen's细胞中乙酰化组蛋白H2B荧光强度较正常对照组减弱,Western blot结果显示噪声组豚鼠耳蜗基底膜中乙酰化组蛋白H2B的表达(H2BAcK5/β-actin比值为0.310 2±0.083 9)较对照组(0.617 9±0.126)明显下调,两组差异有统计学意义(P<0.01)。结论噪声可导致豚鼠内耳感觉细胞乙酰化组蛋白H2B表达水平下降,乙酰化组蛋白失衡有可能参与了噪声性聋的发生。
关键词
噪声性聋;组蛋白;组蛋白乙酰化酶;组蛋白去乙酰化酶;毛细胞
基金项目(Foundation):
国家自然科学基金资助(81300831)
作者
杨登化;龚树生;陈丽艳;谢静;
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