脉冲噪声暴露后大鼠下丘核生长相关蛋白-43表达的研究

Expression of Growth Associated Protein 43 in Inferior Colliculus of Rats at Different Times after Impulse Noise Exposur

张钊;郜元坤;孙晓飞;杨琨;王文静;杨希林;廖华;

1:武汉大学人民医院耳鼻咽喉头颈外科

2:解放军武汉总医院耳鼻咽喉科

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摘要

目的探讨脉冲噪声暴露对大鼠下丘核生长相关蛋白-43(growth associated protein,Gap-43)表达的影响。方法 SPF级成年雄性SD大鼠30只,随机分为正常对照组6只、噪声暴露组24只,正常对照组不接受噪声暴露,噪声暴露组接受脉冲噪声暴露,噪声平均压力峰值156dB SPL,脉宽0.25ms,单次暴露6s,共暴露50次。于噪声暴露前及暴露后3、7、14、28d分别测试对照组及噪声暴露组(每个时间点6只)大鼠听性脑干反应(auditory brainstem response,ABR)阈值;然后取双侧下丘进行免疫组化染色,通过灰度值检测各组大鼠下丘Gap-43蛋白表达水平。结果噪声暴露后各组大鼠ABR反应阈显著高于正常组(P<0.01),噪声暴露后14、28d组ABR阈值显著低于暴露后3d组(P<0.05);噪声暴露后各组大鼠双侧下丘Gap-43蛋白表达显著高于正常组(P<0.01),暴露后28d组大鼠下丘Gap-43蛋白表达显著低于暴露后3d组(P<0.05)。结论脉冲噪声暴露可导致大鼠ABR反应阈显著升高,下丘Gap-43蛋白高表达;脉冲噪声暴露后不同时间大鼠ABR阈值升高和下丘Gap-43蛋白高表达的变化规律可能与听皮层突触重塑有关。

关键词

脉冲噪声;听性脑干反应;下丘核;生长相关蛋白-43

基金项目(Foundation):

国家自然基金面上项目(30772397);; 国家自然科学青年基金(81100712)资助

作者

张钊;郜元坤;孙晓飞;杨琨;王文静;杨希林;廖华;

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脉冲噪声听性脑干反应下丘核生长相关蛋白-43

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