Ca_v1.3钙离子通道在成年大鼠耳蜗的表达

The Expression of Ca_v1.3 Calcium Channels in Adult Rat Cochlea

陈金;周良强;褚汉启;陈请国;杜智会;祁繁;刘云;孙彦博;李鹏军;冰丹;

1:华中科技大学同济医学院附属同济医院耳鼻咽喉头颈外科

摘要

目的观察Ca_v1.3钙离子通道蛋白在成年大鼠耳蜗组织中的表达和分布,探讨其在听觉生理和病理中的作用。方法 35只成年SD大鼠随机分为A组(5只,分离耳蜗冰冻切片)、B组(10只,取耳蜗全部软组织及肾组织)、C组(20只,分离耳蜗基底膜、血管纹及螺旋神经节),采用免疫荧光染色方法检测Ca_v1.3钙离子通道蛋白在耳蜗组织中的分布,采用免疫印迹技术(Western blot,WB)以及逆转录聚合酶链反应(reverse transcriptionpolymerase chain reaction,RT-PCR)检测Ca_v1.3钙通道蛋白在耳蜗组织中的表达。结果免疫荧光显示Ca_v1.3钙通道蛋白主要分布在成年大鼠耳蜗毛细胞、血管纹细胞,螺旋神经板缘细胞、螺旋韧带、螺旋神经节、螺旋凸;RT-PCR以及Western blot的结果显示Ca_v1.3钙离子通道蛋白在耳蜗组织和肾脏中有均表达,其中耳蜗基底膜上最多,螺旋神经节次之,耳蜗侧壁血管纹的表达量与前两者相比相对较少。结论本研究初步揭示了Ca_v1.3钙离子通道蛋白在成年SD大鼠耳蜗中的表达具有一定的组织特异性,为进一步研究Ca_v1.3钙离子通道蛋白在听觉生理和病理中重要的作用提供了理论依据。

关键词

耳蜗;Cav1.3钙离子通道;大鼠

基金项目(Foundation):

国家自然科学基金(81500791,81070787,81271078);; 湖北省卫生计生委科研基金(WJ2015MB062)资助

作者

陈金;周良强;褚汉启;陈请国;杜智会;祁繁;刘云;孙彦博;李鹏军;冰丹;

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耳蜗 Cav1.3钙离子通道大鼠

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陈金周良强褚汉启陈请国杜智会祁繁刘云孙彦博李鹏军冰丹