Nrf2及其信号通路相关因子在噪声性聋大鼠耳蜗的表达

Expression of Nrf2 and Its Related Factors in the Cochlea of Rats with Noise Induced Hearing Loss

蔡蔚然;郑贵亮;葛萨萨;张国平;周义德;

1:第二军医大学长海医院耳鼻咽喉-头颈外科

2:上海远洋医院耳鼻咽喉科

摘要
目的研究Nrf2/Keapl-ARE信号通路相关因子核因子2(nuclear factor E2related factor 2,Nrf2)、超氧化物歧化酶1(superoxide dismutas 1,SOD1)、血红素加氧酶1(heme oxygenase-1,HO-1)在噪声致聋大鼠耳蜗的表达变化。方法 SD大鼠30只,随机分为噪声组15只和正常对照组15只。正常对照组不给予噪声暴露,噪声组动物给予连续12小时115dB SPL的稳态白噪声暴露,分别于噪声暴露后1、3、7天对两组动物进行ABR和DPOAE检测,并于噪声暴露7天后取耳蜗组织运用RT-PCR技术及Peggy Sue微量蛋白检测技术检测Nrf2、SOD1、HO-1的表达。结果噪声暴露后1、3、7天,噪声组大鼠ABR反应阈均高于正常对照组(P<0.05),噪声组DPOAE幅值较正常组明显下降(P<0.05);噪声暴露后7天,噪声组大鼠耳蜗Nrf2、SOD1、HO-1的mRNA表达(1.11±0.05、1.45±0.12、1.15±0.03)上调,高于正常对照组(1.00±0.02、1.10±0.12、0.92±0.08),差异均有统计学意义(P<0.05)。Peggy Sue微量检测系统检测结果显示噪声组Nrf2及SOD1、HO-1蛋白含量均高于正常组(P<0.05)。结论强噪声暴露后,SD大鼠耳蜗Nrf2基因表达和蛋白含量升高,Nrf2/Keapl-ARE信号通路下游抗氧化酶SOD1、HO-1基因表达和蛋白含量也随即上调,此改变可能对噪声引起的耳蜗内氧化应激损伤有一定的保护作用。
关键词
噪声性聋;Nrf2;超氧化物歧化酶1;血红素加氧酶1
基金项目(Foundation):
国家自然科学基金面上项目(81170913);; 国家人力资源和社会保障部留学回国人员科研启动基金(2009年);; 国家教育部留学回国人员科研启动基金联合资助
作者
蔡蔚然;郑贵亮;葛萨萨;张国平;周义德;
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