糖尿病大鼠颈性前庭诱发肌源性电位的观察

Cervical Vestibular Evoked Myogenic Potential in Diabetes Rats

袁艺昕;梁勇;刘小龙;黄雪琴;杨衬;张威;李琦;

1:南方医科大学南方医院耳鼻咽喉头颈外科

2:广东医学院附属医院耳鼻咽喉科

摘要
目的观察糖尿病大鼠不同时期的颈性前庭诱发肌源性电位(cervical vestibular evoked myogenic potential,cVEMP)的变化及意义。方法将220只雄性SD大鼠随机分为对照组(20只)及糖尿病组(200只),应用链脲佐菌素(streptozotocin,STZ)诱导糖尿病大鼠模型,再按照造模的时间将糖尿病组分为糖尿病4、6、8、10、12周组,每组40只,观察各组大鼠的一般情况及血糖、ABR、cVEMP检测结果。结果糖尿病组大鼠造模后出现多饮、多食、多尿等典型糖尿病症状,与对照组比较,血糖升高(P<0.01)、体重下降(P<0.01);糖尿病组从第6周开始出现ABR反应阈值升高(P<0.05);糖尿病组cVEMP阈值第8周(53.87±11.16dB nHL)、第10周(67.00±12.74dB nHL)、第12周(67.00±9.23dB nHL)升高(P<0.01),P1波潜伏期第8周(5.01±0.33ms)、第10周(5.37±0.45ms)、第12周(5.39±0.24ms)延长(P<0.01),N1波潜伏期第10周(8.98±0.86ms)、第12周(9.08±0.45ms)延长(P<0.01),但糖尿病组P1-N1波间振幅与对照组比较差异无统计学意义(P>0.05)。结论糖尿病大鼠从造模成功第8周开始,cVEMP阈值开始升高、P1波及N1波潜伏期延长,出现不同程度的前庭终器损伤,提示cVEMP可作为早期诊断前庭功能损害的方法之一。
关键词
糖尿病;颈性前庭诱发肌源性电位;前庭功能
基金项目(Foundation):
作者
袁艺昕;梁勇;刘小龙;黄雪琴;杨衬;张威;李琦;
参考文献

1Ozel HE,OzkirisM,Gencer ZK,et al.Audiovestibular functions in noninsulin-dependent diabetes mellitus[J].Acta Oto-Laryngologica,2014,134:51.

2 Lindsey M,Tilling TK,Haled D,et a1.Falls as a complication of diabetes mellitus in older people[J].J Diabetes Complications,2006,20:158.

3 Agrawal Y,Carey JP,Della Santina CC,et al.Diabetes,vestibular dysfunction,and falls:analyses from the national health and nutrition examination survey[J].Otol Neurotol,2010,31:1445.

4 Rauch SD.Vestibular evoked myogenic potentials[J].Curt Opin Otolaryngol Head Neck Surg,2006,14:299.

5 Konukseven O,Polat SB,Karahan S,et al.Electrophysiologic vestibular evaluation in type 2diabetic andprediabetic patients:air conduction ocular and cervical vestibular evoked myogenic potentials[J].Int J Audiol,2015,54:536.

6 Yumuk Z,Kucukb asmaci O,Buyukbaba Boral O,et al.The effects of streptozotocin-induced diabetes on brucellosis of rats[J].FEMS Immunol Med Microbiol,2003,39:275.

7 Sakakura K,Miyashita M,Chikamatsu K,et al.Tone burst-evoked myogenic potentials in rat neckextensor and flexor muscles[J].Hear Res,2003,185:57.

8 Yang TH,Young YH.Click-evoked myogenic potentials recorded on alert guinea pigs[J].Hear Res,2005,205:277.

9 Kamali B,Hajiabolhassan F,Fatahi J,et al.Effects of diabetes mellitus typeΙwith or without neuropathyon on vestibular evoked myogenic potentials[J].Acta Med Iran,2013,51:107.

10 Perez R,Ziv E,Freeman S,et a1.Vestibular end-organ impairment in an animal model of type 2diabetes mellitus[J].Laryngoscope,2001,111:110.

11 李鹃,张天宇,沈建中,等.糖尿病患者前庭功能的改变及其临床意义[J].临床耳鼻咽喉头颈外科杂志,2008,22:10.

12 王博琛,梁勇,刘小龙,等.量化的胸锁乳突肌表面肌电对颈部前庭诱发肌源性电位的影响[J].中华耳鼻咽喉头颈外科杂志,2013,48:470.

13 Bektas D,Gazioglu S,Arslan S,et al.VEMP responses are not affected in non-insulin-dependent diabetes mellituspatients with or without polyneuropathy[J].Acta Otolaryngol,2008,128:768.

14 Matsuzaki M,Murofushi T.Click-evoked potentials on the neck of the guinea pig[J].Hear Res,2002,165:152.

15 Myers SF.Myelin-sheath abnormalities in the vestibularnerves of chronically diabetic rats[J].Otolaryngol Head NeckSurg,1998,119:432.

16 Myers SF,Ross MD,Jokelainen P,et a1.Morphological evidence of vestibularpathology in long-term experimental diabetes mellitus.I.Microvascular changes[J].Acta Otolaryngol,1985,100:351.

17 张萍,梁传余,郑芸,等.前庭诱发电位[J].听力学及言语疾病杂志,2004,12:197.

18 吴子明,张素珍,周娜,等.前庭诱发的肌源性电位临床应用[J].中华耳科学杂志,2006,4:298.