正常成年Wistar大鼠短声及短纯音听性脑干反应波形分析

The Wave Analysis of Auditory Brainstem Responses in Normal Adult Wistar Rat

张超;李凤娇;王敏姣;赵艳;黄国威;杨驹;于宁;房琳;郭维维;薛希均;纵亮;关静;王秋菊;

1:中国人民解放军总医院解放军耳鼻咽喉研究所

2:昆明医科大学昆明总医院临床医院耳鼻咽喉科

摘要
目的分析正常Wistar大鼠短声(click)及短纯音(tone burst,TB)诱发听性脑干反应(cABR、tbABR)结果,标准化Wistar大鼠ABR测试方法,为大鼠听觉研究提供参考。方法正常成年雄性Wistar大鼠15只,分别进行cABR和tbABR(4、8、12、16、24、32kHz)测试,观察80、50、20dB SPL强度下各波的引出率,以引出率最高的波来判断阈值,同时量取该波的潜伏期和幅值,检测ABR波Ⅱ、Ⅳ振幅I/O曲线。结果 120dB SPL强度下cABR和tbABR波Ⅱ和波Ⅳ最晚消失的比例几乎相同;2cABR阈值为21.83±4.45dB SPL,432kHz频率tbABR阈值分别为24.33±5.37、14.17±4.37、14.33±4.68、16.67±4.22、23.00±5.81、31.00±7.36dB SPL;380dB SPL cABR波Ⅰa、Ⅰb、Ⅱ、Ⅲ、Ⅳ、Ⅴ潜伏期分别为1.76±0.12、2.13±0.11、2.67±0.16、3.49±0.28、4.39±0.29、5.45±0.41ms,tbABR 4kHz波Ⅰa、Ⅰb、Ⅱ、Ⅲ、Ⅳ、Ⅴ潜伏期分别为2.02±0.09、2.88±0.16、3.77±0.25、4.69±0.29、5.78±0.41ms,8kHz分别为1.76±0.07、2.28±0.10、2.63±0.16、3.49±0.21、4.44±0.28、5.48±0.43ms,12kHz分别为1.76±0.08、2.24±0.12、2.61±0.25、3.53±0.25、4.46±0.32、5.52±0.45ms,16kHz分别为1.79±0.10、2.25±0.12、2.70±0.18、3.62±0.27、4.52±0.37、5.61±0.49ms,24kHz分别为1.75±0.09、2.27±0.11、2.67±0.16、3.60±0.27、4.52±0.38、5.60±0.51ms,32kHz分别为1.77±0.10、2.24±0.12、2.64±0.20、3.59±0.34、4.52±0.40、5.61±0.52ms;4I/O曲线示在高强度时波Ⅱ振幅明显高于波Ⅳ,但在低强度时,波Ⅱ振幅与波Ⅳ基本相同,甚至波Ⅳ振幅高于波Ⅱ。结论正常成年大鼠短声及短纯音诱发ABR波Ⅳ出现率最高,低强度声刺激时,波Ⅱ、Ⅳ振幅基本相同,甚至波Ⅳ振幅高于波Ⅱ,可以波Ⅳ最后消失的强度为阈值。
关键词
大鼠;短声;短纯音;听性脑干反应
基金项目(Foundation):
国家重大科学研究计划项目(2014CB943001);; 国家自然科学基金重点国际合作项目(81120108009);国家自然科学基金重点项目(81530032);; 全军“十二五”重点项目(BWS11J026)联合资助
作者
张超;李凤娇;王敏姣;赵艳;黄国威;杨驹;于宁;房琳;郭维维;薛希均;纵亮;关静;王秋菊;
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