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鼓室注射地塞米松拮抗顺铂耳毒性的研究

The Protective Effects of Intratympanic Dexamethasone to Prevent Cisplatin Ototoxicity

李鹏;张奕;符秋养;孟庆翔;谢景华;梁勇;

1:广州市第一人民医院耳鼻咽喉科

2:南方医科大学附属南方医院耳鼻咽喉头颈外科

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摘要

目的探讨鼓室注射地塞米松对顺铂致听觉损伤的保护作用。方法将55只豚鼠分为4组:正常对照组(I组)10只,腹腔注射生理盐水16ml/kg;地塞米松组(II组)15只,经鼓室注射地塞米松10mg/ml;顺铂组(III组)15只,单次腹腔注射顺铂16mg/kg;顺铂+地塞米松组(IV组)15只,经鼓室注射地塞米松10mg/ml,1小时后腹腔注射顺铂16mg/kg。给药前及给药后第7天测试各组豚鼠听性脑干反应(auditory brainstem response,ABR),并检测耳蜗组织丙二醛(malondiadehyde,MDA)含量和超氧化物歧化酶(superoxide dismutase,SOD)活性。结果 IIV组给药前ABR反应阈分别为28.50±4.74、28.67±5.82、26.67±4.88和27.33±5.30dB nHL,差异无统计学意义(P>0.05);给药后I组和II组ABR反应阈分别为29.00±3.94和31.33±5.81dB nHL,给药前后差异无统计学意义(P>0.05);给药后III组和IV组ABR反应阈为55.33±4.81、40.67±3.72dB nHL,均显著高于给药前(P<0.05),但IV组ABR反应阈低于III组(P<0.05)。给药后I组MDA含量和SOD活性分别为2.01±0.07mmol/L和234.10±13.09U/ml,II组分别为2.06±0.09mmol/L和233.20±13.24U/ml,两组比较差异无统计学意义(P>0.05),III、IV组MDA含量分别为5.74±0.17、3.51±0.18mmol/L,显著高于I、II组(均为P<0.05),但IV组MDA含量显著低于III组(P<0.001);III、IV组SOD活性分别为107.90±14.21、162.70±11.25U/ml,明显低于I、II组(均为P<0.05),但IV组的SOD活性显著高于III组(P<0.001)。结论鼓室注射地塞米松对顺铂耳毒性具有一定的拮抗作用。

关键词

顺铂;耳毒性;地塞米松

基金项目(Foundation):

作者

李鹏;张奕;符秋养;孟庆翔;谢景华;梁勇;

参考文献

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李鹏张奕符秋养孟庆翔谢景华梁勇