电针耳穴对年龄相关性听力损失豚鼠听觉中枢β-catenin表达的影响

The Effects of Electroacupuncture at Tinggong and Yifeng on β-catenin Expression in Auditory Center of Guinea Pig with Age-related Hearing Loss

刘淑云;邓力强;杨烨;殷泽登;

1:西南医科大学附属医院耳鼻咽喉头颈外科

2:湖南省郴州市第一人民医院儿童耳鼻喉科

3:西南医科大学基础医学院生物化学教研室

摘要
目的探讨电针耳穴对D-半乳糖所致的年龄相关性听力损失豚鼠模型听觉中枢β-链蛋白(β-catenin)表达的影响。方法取3月龄豚鼠30只,随机分成三组:D-半乳糖模型组、D-半乳糖+电针组和对照组,每组各10只;18月龄豚鼠10只作为自然老化组。D-半乳糖模型组给予D-半乳糖(300mg·kg(-1)·d(-1)·d(-1))颈背部皮下注射,每天1次,连续6周;对照组给予等量生理盐水注射相同部位、相同频次、持续相同时间;D-半乳糖+电针组给予相同剂量的D-半乳糖颈背部皮下注射,30min后给予电针听宫穴和翳风穴治疗15分钟,每日1次,共6周。自然老化组豚鼠常规饲养。上述实验结束后采用蛋白质印迹(Western blot)方法检测四组豚鼠下丘和听皮层β-catenin蛋白的表达变化。结果 1与对照组相比,D-半乳糖模型组和自然老化组豚鼠下丘β-catenin蛋白表达量下降;与D-半乳糖模型组相比,D-半乳糖+电针组下丘β-catenin蛋白表达量增加;2D-半乳糖模型和自然老化组豚鼠听皮层β-catenin蛋白表达量下降,D-半乳糖+电针组其表达量增加。结论β-catenin蛋白可能通过Wnt/β-catenin信号通路,调控细胞生长、分化及凋亡,参与下丘和听皮层的老化过程;电针听宫穴和翳风穴可能通过增加下丘和听皮层β-catenin蛋白表达,经Wnt/β-catenin信号通路延缓豚鼠年龄相关性听力损失的发生。
关键词
电针;年龄相关性听力损失;听觉中枢;β-链蛋白
基金项目(Foundation):
四川省卫生厅课题(070248)和(100237)资助
作者
刘淑云;邓力强;杨烨;殷泽登;
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