不同基因转染方法对小鼠内耳毛细胞转染效率的比较
The Comparative Study of Different Methods for Gene Transfer to Cochlear Hair Cells
陈岩;赖垂金;李雯;李华伟;
1:复旦大学附属眼耳鼻喉科医院
1 Staecker HD,Li BW,O'Malley Jr,et al.Gene expression in the mammalian cochlea:a study of multiple vector systems[J].Acta Otolaryngol,2001,121:157.
2 Holt JR.Viral-mediated gene transfer to study the molecular physiology of the Mammalian inner ear[J].Audiol Neurootol,2002,7:157.
3 Chen Y,Yu H,Zhang Y,et al.Cotransfection of Pax2and Math1promote in situ cochlear hair cell regeneration after neomycin insult[J].Sci Rep,2013,3:2996.
4 Venail FJ,Wang J,Ruel J,et al.Coxsackie adenovirus receptor and alpha nu beta3/alpha nu beta5integrins in adenovirus gene transfer of rat cochlea[J].Gene Ther,2007,14:30.
5 Pietola L,Aarnisalo AA,Joensuu J,et al.HOX-GFP and WOX-GFP lentivirus vectors for inner ear gene transfer[J].Acta Otolaryngol,2008,128:613.
6 Li Duan,Bordet MT,Mezzina M,et al.Adenoviral and adeno-associated viral vector mediated gene transfer in the guinea pig cochlea[J].Neuroreport,2002,13:1295.
7 Shibata SB,Pasquale GDi,Cortez SR,et al.Gene transfer using bovine adeno-associated virus in the guinea pig cochlea[J].Gene Ther,2009,16:990.
8 Stone IM,Lurie DI,Kelley MW,et al,Adeno-associated virus-mediated gene transfer to hair cells and support cells of the murine cochlea[J].Mol Ther,2005,11:843.
9 叶美玲,王少元.基因转染技术及其在白血病研究中的应用[J].医学综述,2010,16:1934.
10 窦立萍,刘军华,王畅,等.电穿孔转染K562细胞的效率研究[J].军医进修学院学报,2008,29:420.
11 Holt JR,Johns DC,Wang S,et al.Functional expression of exogenous proteins in mammalian sensory hair cells infected with adenoviral vectors[J].J Neurophysiol,1999,81:1881.