豚鼠耳蜗Hensen细胞脂滴的性质与分布

The Distribution of the Lipid Droplets within Hensen Cells in the Guinea-pig Cochlea

杨风波;丁大雄;吕萍;程晓婷;任红苗;黄国威;王晓东;刘晨;张悦;丛涛;杨仕明;翟所强;于宁;

1:川北医学院附院耳鼻咽喉科

2:福建医科大学附属第一医院耳鼻咽喉头颈外科

3:中南大学湘雅二医院耳鼻咽喉科

4:解放军总医院耳鼻咽喉头颈外科

摘要
目的探索豚鼠内耳Hensen细胞(Hensen cells,HC)中透明物质的性质及分布。方法 10只豚鼠分离制备好耳蜗单离Hensen细胞后,采用中性脂滴的特异分子探针Bodipy 493/503、苏丹Ⅲ、油红O染色及锇酸再固定四种染色方法对内耳组织进行染色,观察耳蜗不同区域Hensen细胞中脂滴的分布情况。结果豚鼠耳蜗不同区域Hensen细胞内均存在高透亮物质,从顶回至底回其体积逐渐变小,数量逐渐增加,至底回逐渐消失;该物质经Bodipy 493/503分子探针特异标记为绿色,被苏丹Ⅲ染色为橘红色,被油红O染色为宝石红,被锇酸后固定后呈黑色颗粒,证实为脂滴。结论四种染色方法对内耳组织的染色结果均提示内耳Hensen细胞中透明物质为脂类物质。
关键词
豚鼠;耳蜗;Hensen细胞;脂滴
基金项目(Foundation):
国家973计划重大科学研究计划干细胞项目(2012CB967900与2014CB943002);; 军队项目(BWS14B080;JDZYY20132);; 国家自然基金项目(81271081与81470700);; 四川省卫生厅课题(110298);; 北京市项目(PXM2014-178304-000002-00130227)联合资助
作者
杨风波;丁大雄;吕萍;程晓婷;任红苗;黄国威;王晓东;刘晨;张悦;丛涛;杨仕明;翟所强;于宁;
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