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硫酸铜及顺铂鼓室内给药对大鼠内耳铜离子转运蛋白表达的影响

Change of CTR1 Expression after Round Window Niche Copper Sulfate and Cisplatin Infusion

李俐华;刘伟;任基浩;黄芸;王曼;杨春平;朱新华;刘月辉;

1:南昌大学第二附属医院耳鼻咽喉头颈外科

2:中南大学湘雅二医院耳鼻咽喉头颈外科

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摘要

目的了解铜离子转运蛋白1(copper transport protein 1,CTR1)在大鼠内耳的表达情况及硫酸铜和顺铂鼓室给药对CTR1表达的影响。方法 Wistar雄性大鼠24只,随机分为4组,每组6只,Ⅰ组为正常对照组(非处理组);Ⅱ组为双耳圆窗龛放置顺铂溶液(0.5mg/ml)的明胶海绵;Ⅲ组为双耳圆窗龛放置顺铂溶液(1mg/ml)的明胶海绵;Ⅳ组双耳圆窗龛放置硫酸铜溶液(0.02mg/kg)的明胶海绵。采用免疫组织化学技术,对各组耳蜗冰冻切片行CTR1蛋白的免疫组织化学染色,光学显微镜下观察耳蜗组织中CTR1的表达情况;提取各组耳蜗组织总蛋白,应用Western-blot技术检测各组CTR1蛋白表达水平;提取耳蜗组织的总RNA,应用RT-PCR技术检测各组CTR1mRNA的表达水平。结果ⅠⅣ组大鼠的耳蜗各回Corti器、螺旋神经节、血管纹的细胞质和细胞膜上均有CTR1表达,CTR1的平均光密度值呈降低趋势;各组耳蜗组织均有明显的CTR1表达,ⅠⅣ组大鼠的耳蜗各回Corti器、螺旋神经节、血管纹的细胞质和细胞膜上均有CTR1表达,CTR1的平均光密度值呈降低趋势;各组耳蜗组织均有明显的CTR1表达,ⅠⅣ组CTR1蛋白的光密度值分别为0.532±0.031、0.394±0.024、0.234±0.030和0.191±0.015,呈下降趋势,各组间比较差异有统计学意义(P<0.05);各组耳蜗组织均有明显的CTR1mRNA表达,ⅠⅣ组CTR1蛋白的光密度值分别为0.532±0.031、0.394±0.024、0.234±0.030和0.191±0.015,呈下降趋势,各组间比较差异有统计学意义(P<0.05);各组耳蜗组织均有明显的CTR1mRNA表达,ⅠⅣ组光密度值分别为0.508±0.035、0.391±0.022、0.240±0.023和0.186±0.021,呈下降趋势,各组间比较差异均有统计学意义(P<0.05)。结论 CTR1在大鼠内耳有丰富的表达,其表达量可随鼓室内顺铂浓度增高而下降,且鼓室内给予硫酸酮其表达量下降。

关键词

铜离子转运蛋白1;顺铂;硫酸酮;耳蜗

基金项目(Foundation):

作者

李俐华;刘伟;任基浩;黄芸;王曼;杨春平;朱新华;刘月辉;

参考文献

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