斑马鱼侧线神经丘毛细胞发育的研究

The Zebrafish Model for the Study on Hair Cell Development

李雯;何英姿;孙珊;于慧前;李华伟;

1:复旦大学附属眼耳鼻喉科医院

2:复旦大学医学院生物医学研究院

摘要
目的研究斑马鱼后部侧线系统神经丘细胞早期迁移及增殖分化过程。方法选取不同发育阶段的野生型斑马鱼胚胎,采用免疫组织化学染色和原位杂交方法,观察斑马鱼后部侧线原基的迁徙以及神经丘的沉积过程,BrdU标记原基增殖细胞,观察原基细胞团的增殖情况;选择不同发育时期的转基因斑马鱼(Brn3c:mGFP)胚胎,统计绿色荧光蛋白(GFP)阳性表达的神经丘毛细胞数目;采用活细胞染料FM1-43FX标记神经丘中的功能性毛细胞并进行统计计数,BrdU标记神经丘增殖细胞,观察神经丘细胞的增殖情况。结果受精后20小时的斑马鱼侧线器基板沿水平肌隔开始向后迁移并形成后部侧线神经丘,迁徙速度约为1.7肌节/小时;受精后48小时,初级后部侧线系统基本发育完全,包括躯干部的56组神经丘细胞团,尾部的26组神经丘细胞团,尾部的23组神经丘细胞团;受精后3、5、7天时的斑马鱼神经丘毛细胞数量分别为5.68±1.46、10.10±0.99、12.45±1.32个;FM1-43FX阳性毛细胞数量分别为3.68±1.11、8.18±1.86、10.22±1.24个。结论受精后33组神经丘细胞团;受精后3、5、7天时的斑马鱼神经丘毛细胞数量分别为5.68±1.46、10.10±0.99、12.45±1.32个;FM1-43FX阳性毛细胞数量分别为3.68±1.11、8.18±1.86、10.22±1.24个。结论受精后37天是斑马鱼侧线神经丘毛细胞发育的活跃时期。
关键词
斑马鱼模型;侧线神经丘;毛细胞;发育
基金项目(Foundation):
973及国家重大研究计划(2011CB504506,2010CB945503);; 国家自然科学基金(No.81230019,81070793);; 教育部创新团队(IRT1010);; 上海市优秀学科带头人(11XD1401300);; 教育部博士点基金博导类计划(20120071110077);; 上海市科技启明星计划(12QA1400500)等项目联合资助
作者
李雯;何英姿;孙珊;于慧前;李华伟;
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