长期注射水杨酸钠大鼠ABR及下丘谷氨酸脱羧酶-67表达的变化

Effects of Long Term Injection of Sodium Salicylate on the ABR and Expression of GAD67 in Rat Inferior Colliculus

吴莎;华清泉;杨琨;肖伯奎;张志敏;

1:武汉大学人民医院耳鼻咽喉-头颈外科

摘要

目的观察长期注射水杨酸钠对大鼠听性脑干反应(ABR)及下丘谷氨酸脱羧酶-67(GAD67)表达的影响。方法将18只成年健康Wistar大鼠随机分为水杨酸钠组、生理盐水组、对照组,每组6只。水杨酸钠组肌肉注射10%水杨酸钠175mg/kg,2次/天,连续28天;生理盐水组每天相同时间注射等量生理盐水;对照组不注射任何药物。造模结束后,使用美国TDT系统对三组大鼠进行ABR反应阈及波Ⅲ潜伏期检测,然后将大鼠断头处死并取脑,剥离下丘。采用蛋白质印迹(Western blot)方法检测下丘GAD67蛋白表达的变化。结果①水杨酸钠组、生理盐水组、对照组ABR反应阈分别为61.67±4.08、33.33±2.58、35.83±2.04dB SPL,波Ⅲ潜伏期分别为3.97±0.28、3.51±0.11、3.52±0.09ms。水杨酸钠组较生理盐水组、对照组ABR反应阈明显升高,波Ⅲ潜伏期明显延长(P<0.01),而生理盐水组与对照组差异无统计学意义(P>0.05);②水杨酸钠组下丘GAD67蛋白表达水平明显高于生理盐水组、对照组(P<0.01),而生理盐水组与对照组下丘GAD67蛋白表达水平差异无统计学意义(P>0.05)。结论长期注射水杨酸钠可导致大鼠下丘GAD67蛋白表达水平升高,GAD67蛋白表达的上调可能作为一种负反馈以代偿去抑制所引起的兴奋与抑制的失衡状态,促进抑制性效应的产生,该蛋白表达的改变极有可能作为机体对水杨酸钠耳毒性的抑制性代偿反应和调节机制之一。

关键词

水杨酸钠;听性脑干反应;谷氨酸脱羧酶-67;下丘

基金项目(Foundation):

国家自然科学基金青年基金(81100712)资助

作者

吴莎;华清泉;杨琨;肖伯奎;张志敏;

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吴莎华清泉杨琨肖伯奎张志敏