炎症对声带间充质干细胞生物学行为的影响

The Influence of Inflammation on the Vocal Fold Mesencyhmal Stem Cells Biological Behaviors

杨润琴;梁媛媛;邓志宏;

1:第四军医大学西京医院耳鼻咽喉头颈外科

摘要

目的研究炎症对声带间充质干细胞(vocal fold mesenchymal stem cells,VF-MSCs)生物学行为的影响,为炎症声带损伤修复提供一定的理论依据。方法正常和慢性炎症声带组织分别取自于下咽癌喉全切除的正常声带组织及水肿型声带息肉手术患者切除的标本,所取组织大小约1mm×1mm,每种组织3例,对其行HE、Masson三色染色和Elastin Van Gieson(EVG)染色,观察炎性声带组织较正常组织胶原纤维和弹性纤维改变情况;通过细胞克隆形成实验和MTT细胞生长曲线实验研究炎症对VF-MSCs增殖能力的影响;通过VF-MSCs定向向成骨和成脂细胞分化,研究炎症对VF-MSCs多向分化能力的影响。结果 Masson三色和EVG染色结果显示炎性声带组织固有层细胞外基质(extracellular matrix,ECM)胶原纤维变粗,含量增多,弹力纤维变细,含量减少;细胞克隆形成实验和MTT细胞生长曲线结果显示炎性声带间充质干细胞(inflammatory vocal fold mexenchymal stem cells,I-VF-MSCs)较正常声带间充质干细胞(normal vocal fold mesenchymal stem cells,N-VFMSCs)增殖能力增强;细胞成骨和成脂分化实验结果显示I-VF-MSCs较N-VF-MSCs多向分化能力减弱。结论炎性声带组织较正常声带组织弹性减弱,抗压力增强,炎症促进VF-MSCs增殖能力,抑制VF-MSCs多向分化能力。

关键词

炎症;细胞外基质;声带间充质干细胞;增殖;分化

基金项目(Foundation):

国家自然科学基金(81271065)资助

作者

杨润琴;梁媛媛;邓志宏;

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炎症细胞外基质声带间充质干细胞增殖分化

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杨润琴梁媛媛邓志宏