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慢性锰中毒对大鼠听功能及耳蜗细胞的影响

Effects of Chronic Manganism on Hearing and Cochlear Cells in Rats

唐晓旭;丁忠家;王人凤;施泽涛;邢蔚;闫辉;武瑾;宋勇莉;卢连军;

1:第四军医大学西京医院耳鼻咽喉头颈外科

2:解放军后勤学院门诊部

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摘要

目的探讨锰中毒对大鼠听功能及耳蜗毛细胞、螺旋神经节细胞的影响。方法选取60只断乳21天的SD大鼠,随机分为染毒组和对照组,染毒组(30只)给予氯化锰水溶液(MnCl2·4H2O,100mg·kg-1d-1)连续灌胃12w,对照组(30只)以同法给予等量去离子水。于灌胃4、8和12w后分别检测两组大鼠血锰浓度,于灌胃12w对两组大鼠分别行ABR检测,基底膜铺片鬼笔环肽-异硫氰酸荧光素(Phalloidin-FITC)染色观察毛细胞形态及计数,HE染色观察螺旋神经节细胞形态及计数,透射电镜观察毛细胞及螺旋神经节细胞超微结构的变化。结果染毒组大鼠灌胃4、8、12w后血锰浓度分别为24.1±2.4、31.5±2.6、26.7±2.8ng/ml,明显高于对照组(分别为8.5±1.7、11.2±2.1、12.7±2.1ng/ml),差异有统计学意义(均为P<0.05)。灌胃12w后,染毒组大鼠4、8、16、24、32kHz ABR反应阈均明显高于对照组(P<0.05),其中高频阈值升高显著。灌胃12w后,染毒组耳蜗毛细胞缺失,以底回缺失明显;螺旋神经节细胞数量减少;毛细胞和螺旋神经节细胞线粒体嵴断裂或消失,呈空泡样变。结论慢性锰中毒可导致大鼠耳蜗毛细胞缺失和螺旋神经节细胞减少,进而导致大鼠听功能损伤。

关键词

锰中毒;毛细胞;螺旋神经节细胞;听力损失

基金项目(Foundation):

国家自然科学基金项目(H2602H2607)

作者

唐晓旭;丁忠家;王人凤;施泽涛;邢蔚;闫辉;武瑾;宋勇莉;卢连军;

参考文献

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