快速老化小鼠听功能及耳蜗组织神经生长因子受体p75的增龄性变化

Degenerative Changes of Auditory Function and Age-Related Expression of NGFR p75 in the Cochlea of the Senescence Accelerated Mouse

王杰;刘强和;王翔;张红佳;

1:桂林医学院附属医院耳鼻咽喉-头颈外科

2:桂林医学院耳鼻咽喉科学研究所

摘要
目的探讨快速老化小鼠的听功能及耳蜗组织神经生长因子受体p75(nerve growth factor receptor p75,NGFR p75)表达的增龄性变化特点。方法对3、5、7月龄的快速老化小鼠亚系8(SAMP8小鼠)行8kHz短纯音听性脑干反应(ABR)检测,并用免疫组化染色方法检测耳蜗组织NGFR p75的表达,观察随月龄的增长其ABR反应阈和NGFR p75的表达变化。结果 SAMP8小鼠ABR反应阈值随着月龄增加而显著增高(P<0.05);NGFR p75在不同月龄SAMP8小鼠耳蜗螺旋神经节和毛细胞中均有表达,其光密度值随着月龄增加而显著增高(P<0.05)。结论快速老化小鼠NGFR p75的表达水平随着听觉功能的年龄相关性减退而增高,说明NGFR p75可能与耳蜗功能增龄性退化有关。
关键词
快速老化小鼠;耳蜗;神经生长因子受体p75;听性脑干反应
基金项目(Foundation):
国家自然科学基金(81060083);; 广西科学研究与技术开发计划项目(桂科攻10124001B-56)联合资助
作者
王杰;刘强和;王翔;张红佳;
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