大鼠听皮层发育关键期胶质纤维酸性蛋白表达及听功能变化

Expression of Glial Fibrillary Acidic Protein and Changes of Auditory during Critical Period of Development in the Auditory Cortex in Rats

张志敏;华清泉;杨琨;吴莎;

1:武汉大学人民医院耳鼻咽喉-头颈外科

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摘要

目的观察大鼠听觉发育关键期听功能的变化及听皮层星形胶质细胞标记物胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)的表达。方法清洁级SD大鼠幼鼠50只随机分成出生后第14、21、28、35和42天组,每组10只,分别检测各组大鼠ABR反应阈后,采用免疫组化染色方法及Western blot检测各组大鼠听皮层GFAP的表达。结果大鼠出生后第14、21、28、35、42天组ABR平均反应阈分别为84.5±4.97、70.5±3.69、58.5±5.80、37.0±4.83和35.5±3.69dB SPL,除第35天组与第42天组之外,其余各组两两之间比较差异均有统计学意义(P<0.05或P<0.01);第14、21、28、35、42天组大鼠听皮层GFAP平均累积光密度值(IOD)分别为474.36±234.56、1 465.93±474.96、2 163.06±353.36、6 572.01±808.88和7 244.37±932.90,各组间两两相比差异均有显著统计学意义(P<0.05或P<0.01)。第14、21、28、35和42天组大鼠听皮层GFAP蛋白表达水平分别为1.00±0.06、3.07±0.07、4.92±0.05、6.88±0.03和8.92±0.04,各组间两两相比差异有统计学意义(P<0.05)。结论大鼠出生后第1442天其ABR反应阈逐渐下降,GFAP表达逐渐增强,提示星形胶质细胞可能有促进听皮层及听觉中枢的发育与成熟的作用。

关键词

星形胶质细胞;胶质纤维酸性蛋白;听皮层;发育;大鼠

基金项目(Foundation):

湖北省自然科学基金面上项目(2012FKB04448)资助

作者

张志敏;华清泉;杨琨;吴莎;

参考文献

1 Games KD,Winer JA.Layer V in the rat auditory cortex:projections to the inferior colliculus and cintralateral cortex[J].Hear Res,1988,34:1.

2 Nakahara H,Zhang LI,Merzenich MM.Specialization of primary auditory cortex processing by sound exposure in the critical period[J].Proc Natl Acad Sci USA,2004,101:7170.

3 Zhang LI,Bao S,Merzenich MM.Disruption of primary auditory cortex by synchronous auditory inputs during a critical period[J].Proc Natl Acad Sci USA,2002,99:2309.

4 于常海,赵睿.承载期望的“星”:星形胶质细胞[J].科学(上海),2007,59:13.

5 Eng LF,Vanderthaeghen JJ,Bignami A,et al.An acidic Protein isolated from fibrous astrocytes[J].Brain Res,1971,28:351.

6 薛庆善,郭畹华.大鼠大脑皮质星形胶质细胞的体外培养及其促神经突起生长作用研究[J].神经解剖学杂志,1996,02:151.

7 Azcoitia I,Santos-Galindo M,Arevalo MA,et al.Role of astroglia in the neuroplastic and neuroprotective actions of estradiol[J].Eur J Neurosci,2010,32:1995.

8 Magistretti PJ.Neuroglial metabolic coupling and plasticity[J].Exp Physiol,2011,96:407.

9 Gamrani H,Elgot A,El Hiba O,et al.Cellular plasticity in the supraoptic and paraventricular nuclei after prolonged dehydration in the desert rodent Meriones Shawi:Vasopressin and GFAP immunohistochemical study[J].Brain Res,2011,1375:85.

10 Henneberger C,Rusakov DA.Synaptic plasticity and Ca2+signalling in astrocytes[J].Neuron Glia Biol,2010,6:141.

11 包新民,舒斯云.大鼠脑立体定位图谱[M].北京:人民卫生出版社,1991.34~38.

12 Hensch TK.Critical period regulation[J].Annu Rev Neurosci,2004,27:549.

13 Bures Z,Grecova J,Popelar J,et al.Noise exposure during early development impairs the processing of sound intensity in adult rats[J].Eur J Neurosci,2010,32:155.

14 Leibovici M,Safieddine S,Petit C.Mouse models for human hereditary deafness[J].Curr Top Dev Biol,2008,84:385.

15 Dong Y,Benveniste EN.Immune function of astroeytes[J].Glia,2001,36:180.

16 朱长庚.星形胶质细胞[J].解剖学报,1990,21:441.

17 Prfieger FW,Barres BA.Synaptic efficacy enhanced by glial cells in vitro[J].Science,1997,277:1684.

18 Ullian E,Sapperstein SK,Christopherson KS,et al.,Control of synapse number by glia[J].Science,2001,291:657.

19 谭来勋,孙圣刚,段申汉,等.大鼠海马星形胶质细胞对突触可塑性的影响[J].卒中与神经疾病,2005,12:158.

20 Stallcup WB,Beasley L.Bipotential glial precursor cells of the optic nerve express the NG2proteoglycan[J].J Neurosci,1987,7:2737.

21 寇盛斌,吕来清,潘三强,等.大鼠在学习记忆时内侧隔核星形胶质细胞GFAP表达的变化[J].解剖学研究,2004,26:245.

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