噪声对小鼠耳蜗外侧壁缝隙连接蛋白Connexin26表达的影响

Expression of Connexin26 in Mouse Cochlea Lateral Wall after Noise Exposure

王亚菲;王洁;朱庆春;邱建华;

1:南京军区杭州疗养院海勤疗养区

2:陕西西安市儿童医院耳鼻咽喉头颈外科

3:第四军医大学西京医院全军耳鼻咽喉头颈外科中心

摘要
目的观察噪声性聋小鼠耳蜗外侧壁缝隙连接蛋白Connexin26(Cx26)表达的变化。方法成年雄性Balb/c小鼠49只随机分为噪声组(29只)和对照组(20只)。实验前两组小鼠检测听性脑干反应(ABR),随后噪声组小鼠给予高强度噪声(115dB SPL,6小时/天,共2天12小时)暴露,并在噪声暴露后0和7天分别检测ABR。对照组不做任何处理。噪声暴露后4小时,每组取2只小鼠做耳蜗冰冻切片,18只小鼠提取耳蜗外侧壁总RNA。通过免疫组化染色法观察小鼠耳蜗外侧壁Cx26蛋白的表达,共聚焦显微镜下观察缝隙连接蛋白转运相关蛋白———β微管蛋白的表达,荧光实时定量PCR检测小鼠耳蜗外侧壁Cx26编码基因GJB2mRNA的表达。结果正常小鼠耳蜗螺旋韧带可见Cx26棕黄色阳性颗粒,血管纹处未见阳性表达;噪声组小鼠耳蜗外侧壁Cx26免疫组化染色较对照组减弱;Cx26编码基因GJB2mRNA表达量低于对照组,β微管蛋白排列稀疏紊乱,呈条索状排列,但未见明显浓集或断裂。结论噪声可下调小鼠耳蜗外侧壁Cx26的表达,Cx26可能参与了噪声性聋的发病机制。
关键词
噪声;耳蜗;Connexin26;微管蛋白;小鼠
基金项目(Foundation):
国家自然科学基金资助(81100713)
作者
王亚菲;王洁;朱庆春;邱建华;
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噪声耳蜗Connexin26微管蛋白小鼠

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王亚菲王洁朱庆春邱建华