不同剂量放射线对小鼠畸变产物耳声发射和外毛细胞Prestin蛋白表达的影响
Experimental Observation of Influence of Different Doses of Radiation on Distortion Product Otoacoustic Emission and the Prestin Protein Expression in Mouse Outer Hair Cells
赵向东;梁勇;任陈;袁亚维;
1:南方医科大学南方医院耳鼻咽喉头颈外科
2:南方医科大学南方医院放射治疗科





1 Lau SK,Wei WI,Sham JS,et al.Early changes of auditory brain stem evoked response after radiotherapy for nasopharyn-geal carcinoma--a prospective study[J].J Laryngol Otol,1992,106:887.
2 Jereczek-Fossa BA,Zarowski A,Milani F,et al.Radiother-apy-induced ear toxicity[J].Cancer Treat Rev,2003,29:417.
3 Low WK,Tan MG,Chua AW,et al.12th Yahya Cohen Me-morial Lecture:The cellular and molecular basis of radiation-induced sensori-neural hearing loss[J].Ann Acad Med Sin-gapore,2009,38:91.
4 Zheng J,Shen W,He DZ,et al.Prestin is the motor protein of cochlear outer hair cells[J].Nature,2000,405:149.
5 Schanzler M,Fahlke C.Anion transport by the cochlear motor protein prestin[J].Physiol,2012,590:259.
6 Nilsen N,Brownell WE,Sun SX,et al.Effect of membrane mechanics on charge transfer by the membrane protein prestin[J].Biomech Model Mechanobiol,2012,11:107.
7 Brownell WE,Spector AA,Raphael RM,et al.Micro-and nanomechanics of the cochlear outer hair cell[J].Annu Rev Biomed Eng,2001,3:169.
8 Cheatham MA,Huynh KH,Gao J,et al.Cochlear function in Prestin knockout mice[J].J Physiol,2004,560:821.
9 Oghalai JS.The cochlear amplifier:augmentation of the trave-ling wave within the inner ear[J].Curr Opin Otolaryngol Head Neck Surg,2004,12:431.
10 Zheng J,Anderson CT,Miller KK,et al.Identifying compo-nents of the hair-cell interactome involved in cochlear ampli-fication[J].BMC Genomics,2009,10:127.
11 Wu X,Gao J,Guo Y,et al.Hearing threshold elevation pre-cedes hair-cell loss in prestin knockout mice[J].Brain Res Mol Brain Res,2004,126:30.
12 Liberman MC,Gao J,He DZ,et al.Prestin is required for electromotility of the outer hair cell and for the cochlear am-plifier[J].Nature,2002,419:300.
13 Soh JM,D'Souza VD,Sarepaka GK,et al.Cochlear implant outcomes:a comparison between irradiated and non-irradia-ted Ears[J].Clin Exp Otorhinolaryngol,2012,51:93.
14郭运凯,杨新明,谢鼎华,等.鼻咽癌患者放疗后引起感音神经性聋的临床观察[J].中华耳鼻咽喉头颈外科杂志,2005,40:805.
15 Upadhya IN,Jariwala N,Datar J.Ototoxic effects of irradia-tion[J].Indian J Otolaryngol Head Neck Surg,2011,63:151.
16 Rasmussen R,Claesson M,Stangerup SE,et al.Fractiona-ted stereotactic radiotherapy of vestibular schwannomas ac-celerates hearing loss[J].Int J Radiat Oncol Biol Phys,2012,83:607.
17 Petsuksiri J,Sermsree A,Thephamongkhol K,et al.Senso-rineural hearing loss after concurrent chemoradiotherapy in nasopharyngeal cancer patients[J].Radiat Oncol,2011,6:19.
18 Kim CS,Shin SO.Ultrastructural changes in the cochlea of the guinea pig after fast neutron irradiation[J].Otolaryngol Head Neck Surg,1994,110:419.
19范静平,陆书昌.γ射线与噪声对内耳损伤的实验研究[J].中华耳鼻咽喉科杂志社,1992,27:153.
20 Akmansu H,Eryilmaz A,Korkmaz H,et al.Ultrastructural and electrophysiologic changes of rat cochlea after irradiation[J].Laryngoscope,2004,114:1 276.
21范静平,陆书昌,胡正元,等.急性放射性损伤豚鼠耳蜗扫描电镜观察[J].第二军医大学学报,1992,13:47.
22张孝文,张承发,张建平.头颈部60 Co照射豚鼠耳蜗毛细胞DNA损伤的检测[J].中华耳鼻咽喉科杂志,1998,33:45.
23杨新明,卢永德,陈忠,等.电离辐射对内耳形态与功能影响的实验研究[J].中国耳鼻咽喉颅底外科杂志,1997,3:81.
24陆雪官,刘志勇,张力元,等.鼻咽癌放射治疗后感音神经性耳聋的临床分析[J].中华放射医学与防护杂志,2005,25:264.