弥漫性脑外伤大鼠听功能及耳蜗形态学改变的实验研究

The Experimental Study on the Changes of Hearing Function and Cochlear Morphology in Rat Diffuse Brain Injury Model

邓艳丽;张再兴;刘建新;付兴华;李倩;刘颖;张萍;顾洪兰;卢鹤翔;孙瑞军;

1:河北联合大学附属医院耳鼻咽喉科

2:河北联合大学附属医院重症医学科

3:河北联合大学实验中心电镜室

4:河北联合大学附属医院肿瘤科

摘要
目的观察弥漫性脑外伤(diffuse brain injury,DBI)大鼠听功能和耳蜗形态学的变化。方法 SD大鼠150只,随机分为正常对照组和DBI后1、2、3、4周组,每组30只。对照组不作任何处理,其他4组制作大鼠DBI模型,造模成功后,分别对各组大鼠进行ABR、40Hz AERP、ASSR测试,并以光镜、电镜观察各组大鼠耳蜗形态学变化。结果 DBI后1周组的ABR、40Hz AERP、ASSR阈值较正常组升高,第2、3周组阈值升高更明显,而在第4周组阈值略降低,DBI各组间及各组与对照组间比较差异均有统计学意义(均P<0.05)。电镜观察发现DBI 1周组耳蜗外毛细胞纤毛倒伏,线粒体嵴断裂或呈空泡变性;DBI 2、3周组外毛细胞纤毛倒伏明显,内线粒体空泡变性明显,而DBI 4周组耳蜗损伤改变减轻。结论弥漫性脑外伤可造成听功能受损及耳蜗超微结构损伤性变化。
关键词
大鼠;弥漫性脑外伤;耳蜗;听性脑干反应;40Hz听觉相关诱发电位;听性稳态反应
基金项目(Foundation):
河北省科技厅科技支撑项目(08276101D-74);; 2012河北省医学科学研究重点项目计划(20120407)
作者
邓艳丽;张再兴;刘建新;付兴华;李倩;刘颖;张萍;顾洪兰;卢鹤翔;孙瑞军;
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