阿米卡星对小鼠内耳水通道蛋白4表达的影响

Effect of Amikacin on Aquaporin 4 Expression in Mice Inner Ear

陈观贵;徐亚丽;毛敏;丘理子;

1:广州医科大学附属第二医院耳鼻咽喉科

摘要

目的观察阿米卡星对小鼠内耳水通道蛋白4(aquaporin 4,AQP4)表达的影响,探讨AQP4在阿米卡星耳毒性机制中的可能作用。方法 60只CBA/CaJ小鼠随机分为实验组和对照组,每组30只,实验组小鼠皮下注射阿米卡星450mg·kg-1·d-1,每天1次,共14d,对照组小鼠皮下注射等量生理盐水。注射4天后应用免疫组化染色分别检测两组小鼠耳蜗AQP4的表达及定位情况,应用蛋白免疫印迹技术及RT-PCR技术检测两组小鼠内耳AQP4表达水平的变化。结果 AQP4在小鼠内耳表达于Corti器的支持细胞。对照组和实验组的AQP4蛋白相对含量分别为0.672±0.074、0.479±0.108,AQP4mRNA相对含量分别为0.701±0.107、0.460±0.080,实验组AQP4的蛋白及mRNA的相对含量均低于对照组,差异有统计学意义(P<0.01)。结论阿米卡星可导致小鼠内耳AQP4蛋白及基因表达的下调,这可能是阿米卡星耳毒性的机制之一。

关键词

阿米卡星;内耳;水通道蛋白4;小鼠

基金项目(Foundation):

广州市医药卫生科技项目(201102A213029)

作者

陈观贵;徐亚丽;毛敏;丘理子;

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阿米卡星内耳水通道蛋白4 小鼠

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陈观贵徐亚丽毛敏丘理子