自体成肌细胞甲杓肌注射治疗创伤性声门关闭不全的实验研究

The Effects of Autologous Skeletal Myoblasts Transplantation on Hurted Vocal Cord of Paralysis

闫飚;孙敬武;邹嘉平;张磊;

1:江苏省无锡市第三人民医院耳鼻咽喉头颈外科

2:安徽省立医院耳鼻咽喉头颈外科

摘要
目的观察自体成肌细胞甲杓肌注射对创伤性声门关闭不全的疗效。方法将20只成年新西兰大白兔行左侧喉返神经切断并于左侧声带表面划痕(深度超过上皮层到声带肌),建立左侧喉返神经切断、左侧声带创伤后声带疤痕形成的创伤性声门关闭不全的动物模型;同时,分离这20只大白兔胸锁乳突肌成肌细胞,进行体外培养、分离及扩增。12周后,将20只动物随机分为A、B两组,每组10只,A组(成肌细胞注射组)于左侧声带甲杓肌注射成肌细胞0.3ml时,右侧声带不作任何处理作为正常对照;B组(盐水注射对照组)于左侧声带甲杓肌注射等量生理盐水,右侧声带不作任何处理作为正常对照,12周后分别观察两组动物双侧甲杓肌肌纤维直径和声带体积。结果 A组自体成肌细胞左侧甲杓肌注射12周后双侧甲杓肌肌纤维直径及声带容积大小差异无显著统计学意义(P>0.05),B组注射侧甲杓肌肌纤维直径及声带体积明显小于A组(P<0.05)。结论经甲杓肌注射自体成肌细胞能有效地治疗创伤性声门关闭不全。
关键词
成肌细胞;甲杓肌;声带麻痹
基金项目(Foundation):
作者
闫飚;孙敬武;邹嘉平;张磊;
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