犬声带激光损伤模型的建立及意义

The Establishment and Significance of A Canine Vocal Fold Injury Model by Semiconductor Laser

韩鹏;梁媛媛;赵昱;刘阳;杨润琴;邓志宏;

1:第四军医大学西京医院耳鼻咽喉头颈外科中心

摘要

目的建立支撑喉镜下犬声带激光损伤模型,观察其声带损伤愈合的大体形态学及组织学变化,为研究声带损伤后的修复提供动物模型。方法选用中华田园犬5只,4只于支撑喉镜下以半导体激光损伤双侧声带膜部中后1/3,深达甲杓肌,1只犬未行任何处理作为对照组。分别在术后4d、2、4及8w于支撑喉镜下观察声带大体形态改变后,各处死1只犬取声带损伤部位组织,通过HE染色、Masson三色染色、EVG染色、爱先蓝染色及免疫组化染色观察声带固有层胶原、弹力纤维、透明质酸(HA)及纤维连接蛋白(fibronectin)含量及分布的变化。结果术后4d支撑喉镜下见犬双侧声带充血水肿,组织切片示上皮未被覆固有层,损伤处大量炎性细胞侵润,固有层、肌层结构混乱不清;2w时喉镜下见声带充血水肿较前减轻,切片示受损部位上皮完全被覆,炎性细胞较前减少,胶原增生并排列紊乱,弹力纤维含量减少,HA含量下降,纤维连接蛋白含量增加;4w时喉镜下见声带炎症反应基本消失,受损部位表面不规则,瘢痕形成,切片示受损部位固有层被致密的纤维组织所替代,胶原含量持续增加,弹力纤维含量略下降,HA含量持续下降,Fibronectin含量持续增加;8w时喉镜下仍可见声带瘢痕,损伤处局部萎缩,切片示胶原呈粗大条索状,弹力纤维含量趋于稳定,HA含量下降,Fibronectin含量略上升。结论半导体激光可造成犬声带膜部热损伤模型,损伤后声带固有层内大量纤维组织形成,胶原含量上升,弹力纤维含量降低,HA含量降低,为研究声带损伤修复提供了可靠的动物模型。

关键词

声带;动物模型;支撑喉镜;半导体激光损伤

基金项目(Foundation):

国家自然科学基金资助(30973285)

作者

韩鹏;梁媛媛;赵昱;刘阳;杨润琴;邓志宏;

参考文献

1 Hirano S.Current treatment of vocal fold scarring[J].Curr Opin Otolaryngol Head Neck Surg,2005,13:143.

2 Hansen J,Thibeault S.Current understanding and review of the literature:vocal fold scarring[J].J Vioce,2006,20:110.

3 Hirano S,Minamiguchi S,Yamashita M.Histologic charac-terization of human scarred vocal folds[J].J Voice,2009,23:399.

4 Ichiro T,Tomoko T,Diane B,et al.Cell production in in-jured vocal fold:a rat stady[J].Ann Otol Rhinol Laryngol,2006,115:135.

5 Gray SD,Titze IR,Alipour F.Biomechanical and histologic observations of vocal fold fibrous proteins[J].Ann Otol Rhi-nol Laryngol,2000,109:77.

6 Kanemanl S,NakamruaT,Omori L,et al.Regeneration of the vocal fold using autologous mesenchymal stem cells[J].Ann Otol Rhinol Laryngol,2003,112:915.

7 Byung G,Soo GW,Jin SJ,et al.The prevention of vocal fold scarring using autologous adipose tissue-derived stomal cells[J].Cells Tissues Organs,2006,184:198.

8 Sittel C,Eckel H,Eschenburg C.Phonatory results after la-ser surgery for glottic carcinoma[J].Otolaryngol Head Neck Surg,1998,119:418.

9 Yan Y,Olszewski A,Jiang J.Use of lasers in laryngeal sur-gery[J].J Voice,2010,24:102.

10 Benninger S.Laser surgery for nodules and other benign la-ryngeal lesions[J].Curr Opin Otolaryngol Head Neck Surg.2009,17:440.

11 Remijn E,Marres A,Hoogen J.Endoscopic laser treatment in pre-malignant and malignant vocal fold epithelial lesions[J].Laryngol Otol,2002,116:1 019.

12 Kanemal L,Nakamura T,Yamashita M,et a1.Destiny of autologous bone marrow-derived stromal cells implanted in the vocal fold[J].Ann Otol Rhinol Laryngol,2005,114:907.

13 Hertegard S,Cedervall J,Svensson B,et al.Viscoelastic and histologic properties in scarred rabbit vocal folds after mesen-chymal stem cell injection[J].Laryngoscope,2006,116:1 248.

14 Babak J,Dinesh C,Ming Y,et al.Hylan B Gel Restores Structure and Function to Laser-Ablated Canine Vocal Folds[J].Ann Otol Rhinol Laryngol,2008,117:703.

15 Halum SL,Naidu M,Delo DM,Autologous musclestem cells for the treatment of vocal fold paralysis:pilot study[J].Laryngoscope,2007,117:917.

16 Tateya T,Tateya I,Sohn JH.Histological study of acute vocal fold injury in a rat model[J].Ann Otol Rhinol Laryn-gol,2006,115:285.

17 Rousseau B,Hirano S,Scheidt T,Characterization of vocal fold scarring in a canine model[J].Laryngoscope,2003,113:620.

本文信息

PDF(459K)

本文关键词相关文章

声带动物模型支撑喉镜半导体激光损伤

本文作者相关文章

韩鹏梁媛媛赵昱刘阳杨润琴邓志宏