鸡耳蜗EFNA2基因RNAi慢病毒载体的构建及其靶基因离体沉默效率
Construction of RNAi Lentiviral Vector Targeting EFNA2 Gene and Its Effect of Gene Silencing in PAN of Chicken
余佳;向明亮;吴皓;沈晨凌;
1:上海交通大学医学院附属新华医院耳鼻咽喉-头颈外科
2:上海交通大学医学院耳科学研究所
摘要关键词基金项目(Foundation):作者参考文献1 Ryals BM,Rubel EW.Hair cell regeneration after acoustic trauma in adult Coturnix quail[J].Science,1998,240:1 774.
2 Cruz RM,Lambert PR,Rubel EW.Light microscopic evi-dence of hair cell regeneration after gentamycin toxicity in chick cochlea[J].Arch Otolaryngol Head Neck Surg,1987,113:1 058.
3 Xiang ML,Mu MY,Pao X.Reinnervation of regenerated hair cells in the basilar papilla after kanamycine ototoxicity[J].Acta Otolaryngol(Stockh),2000,120:912.
4 Lee KH,Warchol ME.EphrinA2 may play a role in axon guidance during hair cell regeneration[J].Laryngoscope,2005,115:1 021.
5向明亮,吴皓,李蕴,等.鸡卡那霉素中毒后EphrinA2蛋白表达与耳蜗毛细胞神经连接再生及重塑的关系[J].听力学及言语疾病杂志,2009,17:570.
6 Couto LB,High KA.Viral vector-mediated RNA interfer-ence[J].Curr Opin Pharmacol,2010,10:534.
7 Kuenzel T,Mnig B,Wagner H,et al.Neuronal differentia-tion of the early embryonic auditory hindbrain of the chicken in primary culture[J].Eur J Neurosci,2007,25:974.
8 Zareen N,Greene LA.Protocol for culturing sympathetic neu-rons from rat superior cervical ganglia(SCG)[J].Journal of Visualized Experiments,2009,30:23.
9 Bianchi LM,Dinsio K,Davoli K,et al.Lac z Histochemistry and immunohistochemistry reveal ephrin-B ligand expression in the inner ear[J].J Histochem Cytochem,2002,50:1 641.
10 Cramer KS,Karam SD,Bothwell M,et al.Expression of EphB receptors and EphrinB ligands in the developing chick auditory brainstem[J].J Comp Neurol,2002,452:51.
11 Siddiqui SA,Cramer KS.Differential expression of Eph re-ceptors and ephrins in the cochlear ganglion and eighth crani-al nerve of the chick embryo[J].J Comp Neurol,2005,482:309.