脉冲噪声暴露后大鼠听皮层神经颗粒素的表达研究

The Changes of Neurogranin Level in Rat Auditory Cortex after Impule Noise Exposure

孙晓飞;廖华;杨琨;解为全;

1:武汉大学人民医院耳鼻咽喉-头颈外科

2:安徽合肥市第二人民医院耳鼻咽喉-头颈外科

摘要
目的揭示脉冲噪声暴露后不同时期大鼠听皮层神经颗粒素(neurogranin,Ng)表达的变化。方法将50只成年SD大鼠随机分为5组:正常对照组及脉冲噪声暴露后3、7、14、28天组,每组10只。脉冲噪声暴露条件为:平均压力峰值156dB SPL,脉宽为0.25ms,暴露50次,每次间隔6s。于噪声暴露前及噪声暴露后即刻、3、7、14、28天检测各组大鼠ABR反应阈,同时,应用Western blot方法检测各组大鼠听皮层中的Ng的含量。结果与对照组相比,各组大鼠噪声暴露后即刻、3、7、14、28天各频率ABR反应阈均明显提高(P<0.05),噪声暴露后第7天,各频率ABR阈值有所恢复,第14天时趋于稳定;与对照组(0.68±0.08)比较,噪声暴露后第3、7、14天组大鼠听皮层中Ng的含量分别为0.96±0.05、1.11±0.05、0.78±0.04,显著高于对照组,第28天组为0.36±0.03,显著低于对照组,差异均有统计学意义(P<0.05)。结论噪声暴露后,大鼠ABR反应阈明显升高,听皮层Ng的表达呈现一个先上升后降低的过程,提示脉冲噪声刺激对大鼠听觉系统产生了一定持续性的影响,听皮层出现突触可塑性变化。
关键词
脉冲噪声;听皮层;神经颗粒素
基金项目(Foundation):
国家自然科学基金面上项目(30772397)资助
作者
孙晓飞;廖华;杨琨;解为全;
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孙晓飞廖华杨琨解为全