手机微波辐射对豚鼠耳蜗外毛细胞膜电位的影响

Effects of Mobile Phone on Outer Hair Cells Membrane Potential in Guinea Pig Cochlear

冯晓华;龙孝斌;汪建;陈勇挺;

1:广州军区广州总医院耳鼻咽喉科

2:南方医科大学珠江医院耳鼻咽喉头颈外科

摘要
目的探讨手机微波辐射对豚鼠耳蜗外毛细胞膜电位的影响。方法取成年豚鼠20只随机分为对照组和辐射组,每组各10只。对照组不做任何处理;辐射组:距豚鼠右侧耳廓2cm处对辐射组豚鼠进行手机微波辐射,辐射时间分别为1、6、12、24和48h,然后分别于各时间点随机活杀两组中的豚鼠各2只,取出听泡,分离耳蜗外毛细胞,用2 000Hz纯音进行不同强度的声刺激,观察离体单个外毛细胞(OHC)膜电位的变化特征。结果对照组离体单个OHC予2 000Hz、10dB HL纯音刺激时,于第40s细胞内荧光值开始升高,并于第50s达到峰值(1.112u),随后细胞内荧光值开始回落,于第60s至最低值(1.000u)。辐射组微波辐射1小时后,OHC细胞膜的去极化和复极化时间明显增加,并且不能完全去极化和复极化;辐射6和12h后,随着间断的声刺激可见OHC内荧光值缓慢上升,辐射6小时组之毛细胞于第70s荧光值达峰值(1.100u),而辐射12小时组之毛细胞于第90s荧光值达峰值(1.050u),随后整条曲线呈微上升趋势;辐射24和48h后,经过声刺激,曲线呈微上升、微下降波动,无明显峰值及回落。结论长时间的手机微波辐射能导致离体豚鼠耳蜗OHC膜离子通透性的改变,影响OHC膜电位的形成及细胞去极化、复极化,从而导致离体OHC膜电位对不同强度纯音刺激的敏感性逐渐降低。
关键词
手机;微波辐射;豚鼠;外毛细胞;膜电位
基金项目(Foundation):
广东省医学科研基金资助项目(B2010251)
作者
冯晓华;龙孝斌;汪建;陈勇挺;
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