噪声对小鼠耳蜗外侧壁缝隙连接蛋白Connexin31表达的影响

Expression of Connexin31 in Cochlea Lateral Wall of Mouse after Noise Exposure

王亚菲;王娟;张鹏志;米文娟;蒋兴旺;王洁;邱建华;

1:第四军医大学西京医院全军耳鼻咽喉头颈外科中心

摘要

目的观察噪声性聋小鼠耳蜗外侧壁缝隙连接蛋白Connexin31(Cx31)表达的变化。方法选用成年雄性Balb/c小鼠44只,随机分为噪声组和对照组,每组22只。两组小鼠均在实验前检测听性脑干反应(ABR),随后应用声刺激器给予噪声组小鼠高强度白噪声(115dB SPL,6h/d,共2天),并在噪声暴露结束后1h再检测噪声组小鼠ABR。对照组不予噪声刺激。于噪声暴露后4h,每组各取4只小鼠耳蜗做冰冻切片,其余18只小鼠提取耳蜗外侧壁组织总RNA,通过免疫荧光染色法观察小鼠耳蜗外侧壁Cx31蛋白的表达,荧光实时定量PCR检测小鼠耳蜗外侧壁Cx31mRNA的表达。结果对照组小鼠耳蜗螺旋韧带可见Cx31特异性荧光,血管纹处未见阳性荧光;噪声组小鼠耳蜗外侧壁免疫荧光染色阳性反应较对照组减弱,Cx31mRNA表达量明显低于对照组。结论噪声能下调Cx31在小鼠耳蜗外侧壁组织的表达,Cx31可能参与了噪声性聋的发病机制。

关键词

噪声;耳蜗;缝隙连接蛋白;小鼠

基金项目(Foundation):

国家973项目课题(2011CB504505);; 国家自然科学基金海外重大合作项目(81120108008);国家自然科学基金重点项目(30930098)资助

作者

王亚菲;王娟;张鹏志;米文娟;蒋兴旺;王洁;邱建华;

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噪声耳蜗缝隙连接蛋白小鼠

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王亚菲王娟张鹏志米文娟蒋兴旺王洁邱建华