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BMP4/Smad4在C57BL/6小鼠内耳前庭发育不同阶段的表达

Expression of BMP4/Smad4 in C57BL/6 Mice Vestibule at Different Developmental Stages

邓安春;杨仕明;黄德亮;孙建和;

1:第三军医大学新桥医院耳鼻咽喉科

2:解放军总医院耳鼻咽喉头颈外科

3:解放军耳鼻咽喉研究所

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摘要

目的探讨骨形态形成蛋白4(bone morphogenetic protein 4,BMP4)和Smad4蛋白(drosophila mothers against decapentaplegic protein 4)在C57BL/6小鼠内耳前庭发育不同阶段的表达。方法选择从胚胎第10天(E10)到胚胎第20天(E20)每天的孕鼠各两只(共22只),取E10～E17的胚胎头、E18～E20的胚胎内耳,通过冰冻连续切片、免疫组化或免疫荧光染色,观察小鼠胚胎内耳前庭发育过程中BMP4/Smad4的表达情况。结果 BMP4从E10开始,在间充质浓聚前就有表达;间充质一开始浓聚,BMP4在其中就有较丰富的表达;早期主要表达在前庭囊及前庭囊周围的间充质,在胚胎发育的后期,其在软骨囊及前庭上皮里有广泛阳性表达;Smad4在小鼠内耳前庭的表达情况与BMP4不一致,E10到E12的小鼠内耳Smad4表达阴性,E15时各个壶腹嵴、囊斑里才有较明显的Smad4表达,而内耳周围软骨囊到E16时才有明显的Smad4表达。结论 BMP4与小鼠内耳前庭形态形成及毛细胞分化发育、功能形成有关;Smad4与BMP4的表达时空特征不同,Smad4可能主要参与了小鼠内耳前庭发育后期的形态塑形和功能发育。

关键词

小鼠;前庭;BMP4/Smad4

基金项目(Foundation):

国家自然科学基金重点项目(30730040)和面上项目(30571017、30871398);; 重庆市回国人员启动基金资助项目(CSTC,2010BB5032);; 第三军医大学回国人员启动基金资助项目(2010XHG05)

作者

邓安春;杨仕明;黄德亮;孙建和;

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邓安春杨仕明黄德亮孙建和