首页 > 过刊浏览 > 文章详情

尼莫地平对耳蜗缺血再灌注损伤的保护作用

The Protective Effects of Nimodipine on Cochlear Impairment Induced by Ischemia-Reperfusion

夏树前;龚树生;李娇;张富刚;

1:华中科技大学附属协和医院耳鼻咽喉科

2:湖北省阳新县人民医院耳鼻咽喉科

3:首都医科大学附属同仁医院耳鼻咽喉科

在线阅读

摘要

目的观察尼莫地平对豚鼠耳蜗缺血再灌注损伤的保护作用并探讨其作用机制。方法 42只豚鼠随机分为干预组、实验组和正常对照组,干预组和实验组各18只,用FeCl3诱导小脑前下动脉缺血后以尿激酶溶栓制备缺血再灌注模型,干预组在应用FeCl310分钟前腹腔注射尼莫地平0.5 mg/kg,实验组在相同条件下注射等量生理盐水;正常对照组6只,不造模,不注射任何药物。连续记录各组耳蜗血流量,基底膜Hoechest33342荧光染色观察细胞核形态学变化,耳蜗中轴切片免疫组织化学染色检测螺旋神经节Casepase-3的表达。三组均于实验术前检测ABR,实验组、干预组分别于再灌注后1、2、6 h再行ABR检测。结果小脑前下动脉缺血后,实验组和干预组耳蜗血流量明显下降,再灌注后又明显回升,ABR反应阈值较对照组上升,实验组较干预组上升明显,差异有统计学意义(P<0.01)。实验组、干预组均出现内毛细胞缺损,且实验组内毛细胞缺损率较对照组高,差异有统计学意义(P<0.01)。缺血再灌注后,实验组螺旋神经节Casepase-3较干预组表达增强,差异有统计学意义(P<0.01)。结论豚鼠耳蜗缺血再灌注导致内毛细胞和螺旋神经节细胞发生凋亡损伤,尼莫地平对耳蜗缺血再灌注损伤有一定保护作用。

关键词

缺血再灌注;尼莫地平;激光多普勒;凋亡;荧光染色;Casepase-3

基金项目(Foundation):

作者

夏树前;龚树生;李娇;张富刚;

参考文献

1吴昊,周维镕,徐彬.脉鼠耳蜗微循环障碍模型的实验研究[J].南通医学院报,2003,23:246.

2 Tabuchi K,Pak K,Chavez E,et al.Role of inhibitor of apopto-sis protein in gentamicin-induced cochlear hair cell damage[J].Neuroscience,2007,12,149:213.

3 Mizutairi K,Matsunaqa T,Kamiya K,et al.Caspase inhibitorfacilitates recovery of hearing by protecting the cochlear lateralwall fromacute cochlear mitochondrial dysfunction[J].J Neu-rosci Res,2008,86:215.

4 Taniquchi M,Hakuba N,Koqa K,et al.Apoptotic hair celldeath after transient cochlear ischemia in gerbils[J].Neurore-port,2002,13:2 459.

5 Osborn KD,Zaidi A,Mandal A,et al.Single-molecule dy-namics of the calcium-dependent activation of plasma-mem-brane Ca2+-ATPase by cal modulin[J].Biophys J,2004,87:1 892.

6 Rizzuto R,Pozzan T.Microdomains of intracellular Ca2+:Molec-ular determinants and functional consequences[J].Physiol Rev,2006,86:369.

7 Rachel T,Morton-Jones R T.Ca2+entry via AMPA-typeglutamate receptors triggers Ca2+induced Ca2+release fromryanodine receptors in rat spiral ganglion neurons[J].Cell Cal-cium,2008,43:356.

8 Morton-Jones RT,Cannel MB,Jeyakumar LH,et al.Differ-ential expression of ryanodine receptors in the rat cochlea[J].Neuroscience,2006,137:275.

9 Erausquin GA.Transactivation of cell death signals by gluta-mate transmissionin dopaminergic neurons[J].Crit Rev Neu-robiol,2004,16:107.

10 Verkhratsky A.Physiology and pathophysiology of the calci-umstorein the endoplasmic reticulumof neurons[J].PhysiolRev,2005,85:201.

11 Johnson JEJr,Perkins GA,Giddabasappa A.Spatiotemporalregulation of ATP and Ca2+dynamics in vertebrate rod andcone ribbon synapses[J].Mol Vis,2007,13:887.

12 Dietz RM,Kiedrowski L,Shuttleworth CW.Contribution ofNa+/Ca2+exchange to excessive Ca2+loading in dendritesand somata of Ca1 neurons in acute slice Hip[J].Pocampus,2007,17:1 049.

13 Schnee ME,Ricci AJ.Biophysical and pharmacological char-acterization of voltage-gated calciumcurrents in turtle audi-tory hair cells[J].J Physiol,2003,549(Pt 3):697.

14马英蓉,李学佩,赵一鸣,等.尼莫地平对正常和椎基底动脉供血障碍时耳蜗血流的影响[J].临床耳鼻咽喉科杂志,2002,16:174.

本文信息

PDF(413K)

本文关键词相关文章

缺血再灌注尼莫地平激光多普勒凋亡荧光染色 Casepase-3

本文作者相关文章

夏树前龚树生李娇张富刚