葛根素对噪声性聋的防治作用

Protective Efffects of Puerarin against Noise-Induced Hearing Loss in Guinea Pigs

陈鑫;邱建华;

1:第四军医大学西京医院耳鼻喉头颈外科

摘要

目的探讨葛根素对噪声性聋的预防及治疗作用。方法 18只白化雄性豚鼠随机分为三组,每组6只,单纯噪声组每日腹腔注射生理盐水2 ml,噪声+葛根素组每日腹腔注射葛根素150 mg/kg,空白对照组每日腹腔注射生理盐水2 ml。三组动物均持续给药10日。单纯噪声组和葛根素组于给药第3日给予噪声刺激,噪声模式采用4 kHz纯音,强度128 dB SPL,时间6小时。并于实验第1、4、7、10日分别对各组豚鼠进行ABR检测。10日后随机抽取各组豚鼠进行耳蜗基底膜铺片、免疫组化染色、硝基自由基含量测定。结果三组豚鼠噪声刺激前ABR阈值无明显差异(P>0.05);实验第4、7、10日时,噪声+葛根素组ABR反应阈较单纯噪声组显著降低(P<0.01),但比空白对照组明显升高(P<0.01)。耳蜗基底膜铺片结果显示,单纯噪声组外毛细胞缺失率约72.65%,噪声+葛根素组外毛细胞缺失率约21.48%,空白对照组外毛细胞无缺失。免疫组化染色的结果显示,噪声+葛根素组的硝基自由基表达较单纯噪声组弱,但比空白对照组强。结论葛根素对噪声性聋有一定的防治作用,可以减少听力下降的幅度,降低毛细胞损伤的比例,但不能完全逆转噪声所致的听力下降。

关键词

噪声性聋;葛根素;活性氮;自由基

基金项目(Foundation):

作者

陈鑫;邱建华;

参考文献

1 Fetoni AR,Piacentini R,Fiorita A,et al.Water-soluble Co-enzyme Q10 formulation(Q-ter)promotes outer hair cellsurvival in a guinea pig model of noise induced hearing loss(NIHL)[J].Brain Research,2009,1257:108.

2 Yamashita D,Jiang HY,Prell L,et al.Post-exposure treat-ment attenuates noise-induced hearing loss[J].Neuro-science,2005,134:633.

3 Shibata SB,Osumi Y.Administration of amitriptyline attenu-ates noise-induced hearing loss via glial cell line-derivedneurotrophic factor(GDNF)induction[J].Brain Research,2007,1144:74.

4 Coleman JKM,Kopke RD.Pharmacological rescue of noise in-duced hearing loss using N-acetylcysteine and acetyl-L-carnitine[J].Hearing Research,2007,226:104.

5 Ohinata Y,Yamasoba T,Schacht J,et al.Glutathione limitsnoise-induced hearing loss[J].Hearing Research,2000,146:28.

6 Han Y,Zhong CP,Hong L,et al.Effect of c-myc on the ul-trastructural structure of cochleae in guinea pigs with noise in-duced hearing loss[J].Biochemical and Biophysical ResearchCommunications,2009,390:458.

7郑贵亮,翟所强.噪声性耳聋的发病机制研究进展[J].山东医药,2008,48:115.

8 Kaygusuz I,Ozturk A.Role of free oxygen radicals in noise-re-lated hearing impairment[J].Hearing Research,2001,162:43.

9 Ohinata Y,Miller JM,Altschuler RA,et al.Intense noise in-duces formation of vasoactive lipid peroxidation products in thecochlea[J].Brain Research,2000,878:163.

10 Salvi A,Carrupt PA,Tillement JP,et al.Structura damageto proteins caused by free radicals:Asessment,protection byantioxidants,and in uence of protein binding[J].BiochemPharmacol,2001,61:1 237.

11 Halliwell B.Free radicals,antioxidants,and human disease:Curiosity,cause,or consequence[J].Lancet,1994,344:721.

12 Jacono AA,Hu BH,Kopke RD,et al.Changes in cochlearantioxidant enzyme activity after sound conditioning and noiseexposure in the chinchilla[J].Hearing Research,1998,117:31.

13 Yamasoba T,Harris C,Schoji F,et al.In uence of intensesound exposure osynthesis in the cochlea[J].Brain Research,1998,804:72.

14陈贤明,王锦玲,姜鸿彦.一氧化氮对庆大霉素耳毒性的影响[J].中国耳鼻咽喉颅底外科杂志,2002,8:249.

15 Yamashita D,Jiang HY,Schacht J,et al.Delayed produc-tion of free radicals following noise exposure[J].Brain Re-search,2004,1019:201.

16纵亮,王锦玲,冯悦,等.葛根素对急性低气压环境下噪声性听器损伤的防治作用[J].听力学及言语疾病杂志,2004,12:104.

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噪声性聋葛根素活性氮自由基

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陈鑫邱建华