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耳蜗中L-精氨酸对Ca~(2+)-ATP酶抑制剂的拮抗作用

贾学斌;李兴启;张瀛;朱耀国;

1:山西省大同市三医院耳鼻咽喉科

2:中国人民解放军总医院耳鼻喉研究所

3:昆明医学院第一附属医院耳鼻咽喉科

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摘要

目的探讨耳蜗中L-精氨酸对Ca2+-ATP酶抑制剂的拮抗作用。方法选择健康杂色豚鼠70只,雌雄不限,随机分为7组,每组10只:①人工外淋巴液组;②Ca2+-ATP酶抑制剂组;③L-精氨酸组;④Ca2+-ATP酶抑制剂+L-精氨酸组;⑤Ca2+-ATP酶抑制剂+环磷酸鸟苷(cGMP)组;⑥Ca2+-ATP酶抑制剂+L-精氨酸+非选择性一氧化氮合酶(NOS)抑制剂组;⑦Ca2+-ATP酶抑制剂+L-精氨酸+可溶性环磷酸鸟苷合酶sGC抑制剂组。各组动物分别行全耳蜗灌流以上各组药物120分钟,灌流过程中由圆窗龛每隔30分钟测1次耳蜗微音器电位(cochlear microphonic,CM)和听神经复合动作电位(compound action potential,CAP),比较各组结果。结果第3组灌流Ca2+-ATP酶抑制剂前后CAP阈移为28.5 dB,第4组在此基础上加入L-精氨酸可使CAP阈移改善9 dB,与第5组加入cGMP后作用相似;第6组多加入非选择性NOS抑制剂后CAP阈移为29.5 dB,与第7组加入可溶性环磷酸鸟苷合酶sGC抑制剂作用相似。结论①Ca2+-ATP酶抑制剂通过抑制Ca2+-ATP酶使胞内Ca2+浓度升高,可对耳蜗功能产生影响;②L-精氨酸通过激活NO/cGMP通路可对Ca2+-ATP酶抑制剂的作用产生部分拮抗。

关键词

L-精氨酸;Ca2+-ATP酶;Ca2+-ATP酶抑制剂;耳蜗微音电位

基金项目(Foundation):

国家自然科学基金资助(30070812)

作者

贾学斌;李兴启;张瀛;朱耀国;

参考文献

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L-精氨酸 Ca2+-ATP酶 Ca2+-ATP酶抑制剂耳蜗微音电位

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贾学斌李兴启张瀛朱耀国