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豚鼠耳石修复再生的超微结构及SPARC动态变化

Dynamic Changes of Ultrastructure and SPARC of Otoconia during Repair and Regeneration Process in Guinea Pig

杨蒙生;贾宏博;谷京城;刘振华;穆兰;

1:辽宁医学院附属第一医院耳鼻咽喉科

2:空军航空医学研究所

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摘要

目的观察豚鼠前庭耳石器受离心力高过载(高G)损伤后,其功能和形态学的变化及富含半胱氨酸的酸性分泌性糖蛋白(SPARC)在其再生过程中的作用。方法 40只豚鼠随机分为对照组(n=10)和离心力高过载组(高G组,n=30),高G组给予10G离心力刺激4min后,分别于刺激后当天、三天、七天(每组10只)断头处死取双侧椭圆囊行扫描电镜观察,并应用免疫组织化学染色方法观察SPARC在豚鼠前庭中的表达。结果 10G离心力作用5min后,76.67%(23/30)的豚鼠出现了耳石器损伤的阳性行为学症状,且所有阳性症状均在30min内消失。扫描电镜下显示:高过载后当天可见正常耳石形态消失,被大量球状物代替,三天时球状物逐渐矿化,七天时哑铃状耳石出现并与矿化球状物共存,此外各时期均可见各种耳石的变性。免疫组织化学染色发现高G组在10G刺激后不同时间SPARC染色均高于对照组(P<0.05),但高G组各组之间差异无统计学意义(P>0.05)。左右耳未发现差别。结论离心力高过载刺激可造成前庭系统功能紊乱和耳石器结构损害,SPARC与耳石再生有关。

关键词

耳石;SPARC蛋白;超微结构;再生

基金项目(Foundation):

国家自然基金资助(30871220)

作者

杨蒙生;贾宏博;谷京城;刘振华;穆兰;

参考文献

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杨蒙生贾宏博谷京城刘振华穆兰