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声刺激诱发麻醉豚鼠前庭咬肌反射及起源的研究

Click Sound-Induced Reflex Potentials on the Masseter Muscle and Its Origin in Anesthesia Guinea Pig

孙伟;孔维佳;范国润;

1:华中科技大学同济医学院附属协和医院耳鼻咽喉科

2:解放军第209医院耳鼻咽喉科

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摘要

目的建立声诱发前庭咬肌反射的动物模型,探讨咬肌反射电位的特征及起源。方法 20只豚鼠,随机分为正常对照组(10只)、单侧前庭下神经切断组(5只)、单侧耳蜗神经破坏组(5只)。3组动物分别在麻醉下于豚鼠下颌骨及颅顶之间用金属夹夹住,使咬肌保持一定的张力,记录click声诱发的咬肌反射电位,并进行听性脑干反应(ABR)测试。结果正常对照组豚鼠声诱发咬肌反射电位的负波(negativepeak,NP)阈值为92±7.68dBnHL。给予100、90、80、70dBnHL单侧声刺激时,同侧记录咬肌反射NP引出率分别为100%、70%、40%、0%。给予100、90、80dBnHL单侧声刺激时,同侧记录咬肌反射NP平均潜伏期分别为6.57±0.26、6.64±0.23、6.69±0.19ms,不同刺激强度下NP潜伏期差异无统计学意义(P>0.05)。ABR的平均反应阈为31±7.88dBnHL。单侧前庭下神经切断组术侧声诱发咬肌反射消失,ABR反应阈在正常范围内。单侧耳蜗神经破坏组术侧声诱发咬肌反射存在,NP阈值及潜伏期与正常对照组差异无统计学意义(P>0.05),ABR消失。结论在豚鼠下颌骨及颅顶之间应用金属夹使咬肌保持一定的张力,同时给予click声刺激,可以建立一个理想的声刺激诱发前庭咬肌反射的动物模型;声刺激诱发的豚鼠咬肌反射电位NP来源于前庭,且该反射是肌源性的。

关键词

前庭;声刺激;咬肌反射电位;听性脑干反应

基金项目(Foundation):

作者

孙伟;孔维佳;范国润;

参考文献

1Murofushi T,Curthoys IS,Topple AN,et al.Response of guinea pig pri mary vestibular neurons to clicks[J].Exp Brain Res,1995,103:174.

2McCue MP,Guinan JJ.Spontaneous activity and frequency se-lectivity of acoustically responsive vestibular afferents in the cat[J].J Neurophysiol,1995,74:1563.

3Brantberg K,Bergenius J,Tribukait A.Vestibular evoked myo-genic potentials in patients with dehiscence of the superior semicircular canal[J].Acta Otolaryngol,1999,119:633.

4Rauch SD,Silveira MB,Zhou G,et al.Vestibular evoked myo-genic potentials versus vestibular test battery in patients with Meniere’s disease[J].Eur Otol Neurotol,2004,25:981.

5Chen CW,Young YH,Wu CH.Vestibular neuritis:Three-di-mensional videonystagmography and vestibular evoked myo-genic potential results[J].Acta Otolaryngol,2000,120:845.

6Matsuzaki M,Murofushi T,Mizuno M.Vestibular evoked my-ogenic potentials in acoustic tumor patients with normal audi-tory brainstem responses[J].Eur Arch Otorhinolaryngol,1999,256:1.

7Deriu F,Tolu E,Rothwell JC.Ashort latency vestibulomasse-teric reflex evoked by electrical sti mulation over the mastoidin healthy humans[J].J Physiol,2003,553:267.

8Deriu F,Tolu E,Rothwell JC.Asound-evoked vestibulomas-seteric reflexin healthy humans[J].J Neurophysiol,2005,93:2739.

9Tolu E,Pugliatti E.The vestibular system modulates masseter muscle activity[J].J Vestib Res,1993,3:163.

10Satoh Y,Ishizuka KI,Murakami T.Modulation of the masse-teric monosynaptic reflex by sti mulation of the vestibular nu-clear complexin rats[J].Neurosci Lett,2009,466:16.

11Deriu F,Ortu E,Capobianco S.Origin of sound-evoked EMG responses in human masseter muscles[J].J Physiol,2007,580:195.

12Giaconi E,Deriu F,Tolu E.Transneuronal tracing of vestibu-lo-trigeminal pathways innervating the masseter muscle in the rat[J].Exp Brain Res,2006,171:330.

13Cuccurazzu B,Deriu F,Tolu B.A monosynaptic pathwaylinks the vestibular nuclei and masseter muscle motoneuronsin rats[J].Exp Brain Res,2007,176:665.

14Akin FW,Murnane OD,Panus PC,et al.Theinfluence of vol-untary tonic EMGlevel on the vestibular-evoked myogenic potential[J].J Rehabil Res Dev,2004,41:473.

15Miles TS,Flavel SC,Nordstrom MA.Control of human man-dibular posture during locomotion[J].J Physiol,2004,554:216.

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孙伟孔维佳范国润