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高胆红素血症对新生豚鼠畸变产物耳声发射的影响

Effect of Hyperbilirubinemia on Distortion Product Otoacoustic Emissions in Newborn Guinea Pigs

叶海波;时海波;于栋祯;陈正侬;殷善开;

1:上海交通大学附属第六人民医院耳鼻咽喉科

2:上海交通大学耳鼻咽喉科研究所

3:上海交通大学眩晕疾病诊治中心

4:上海市听力测试中心

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摘要

目的通过动态观察高胆红素血症新生豚鼠畸变产物耳声发射(DPOAE)及其对侧抑制现象的变化,探讨高胆红素血症对耳蜗和/或脑干听觉传出通路内侧耳蜗橄榄系统功能的影响。方法 20只新生豚鼠分为实验组和对照组各10只,分别腹腔注射胆红素100μg/g和等量生理盐水。两组于给药前、给药后2、4、6、8小时在对侧耳无声刺激及70dBSPL白噪声刺激两种条件下测试DPOAE,观察两组DPOAE及对侧抑制现象的动态变化。给药后8小时行全耳蜗基底膜铺片定量观察毛细胞损伤情况。结果对照组给药前后DPOAE幅值无变化,对侧抑制现象始终存在。实验组给药后DPOAE幅值逐步下降,给药后2小时起对侧抑制现象消失,其中5只豚鼠在给药后8小时DPOAE幅值及对侧抑制现象呈现不同程度的恢复。两组豚鼠耳蜗基底膜铺片观察毛细胞形态未见异常。结论高胆红素血症早期可能损害耳蜗毛细胞的主动机械活动及耳蜗传出神经系统,此损害部分为可逆性的。DPOAE及其对侧抑制现象是评估胆红素脑病严重程度的有效指标。

关键词

高胆红素血症;耳声发射;对侧抑制;耳蜗传出系统

基金项目(Foundation):

2008年度上海市科委“创新行动计划”基础研究重点项目基金(08JC1412700);; 2007年度教育部留学回国人员科研启动基金资助

作者

叶海波;时海波;于栋祯;陈正侬;殷善开;

参考文献

1Watson RL.Hyperbilirubinemia[J].Crit Care Nurs Clin North AM,2009,21:97.

2Watchko JF.Kernicterus and the molecular mechanisms of bi-lirubin-induced CNSinjury in newborns[J].Neuromolecular Med,2006,8:513.

3Wilkinson AR,Jiang ZD.Brainstemauditory evoked response in neonatal neurology[J].Semin Fet Neonatol Med,2006,11:444.

4Shapiro SM,Nakamura H.Bilirubin and the Auditory System[J].Journal of Perinatology,2001,21:852.

5Shaia WT,Shapiro SM,Spencer RF.The jaundiced Gunn rat model of auditory neuropathy/dyssynchrony[J].Laryngo-scope,2005,115:2167.

6Oysu C,Ulubil A,Aslan I.Incidence on cochlear involvement in hyperbilirubinemic deafness[J].J Ann Otol RhinoLaryngol,2002,111:1021.

7Harrison RV,Sharma A,Brown T.et al.Amplitude modula-tion of DPOAEs by acoustic sti mulation of the contralateral ear[J].Acta Oto-Laryngologica,2008,128:404.

8孔维佳,杨盈,章巍.豚鼠畸变产物耳声发射潜伏期的对侧抑制效应现象[J].中华耳鼻咽喉科杂志,2001,36:271.

9陈舜年,贲晓明,李佩红,等.胆红素脑病动物模型制作与鉴定[J].新生儿科杂志,1997,12:166.

10丁大连,李明,姜泗长,等.内耳形态学[M].哈尔滨:黑龙江科学技术出版社,2001.24~25.

11Ahdab-Barmada M.The neuropathology of kernicterus:Def-initions and debate[M].Maisels MJ,Watchko JF,eds.Neo-natal Jaundice.Amsterdam,The Netherlands:Harwood Aca-demic Publishers,2000.77~88.

12Lafreniere D,Smurzynski J,Jung M.Otoacoustic emissions in full-termnewborns at risk for hearing loss[J].Laryngo-scope,1993,103:1334.

13Sheykholeslami K,Kaga K.Otoacoustic emissions and audi-tory brainstem responses after neonatal hyperbilirubinemia[J].Int J Pediatr Otorhinolaryngol,2000,52:65.

14Shapiro SM,Te Selle ME.Cochlear microphonics in the jaun-diced Gunn rat[J].AmJ Otolaryngol,1994,15:129.

15王洪田,钟乃川.皮层传出调控对畸变产物耳声发射的影响[J].中华耳鼻咽喉头颈外科杂志,1999,34:340.

16Abe T,Tsuiki T,Ito S,et al.Suppression of evoked otoa-coustic emissions by contralateral noise exposure in humans[J].Nippon Jibiinkoka Gakkai Kaiho.1990,93:1890.

17Kujawa SG,Glattke TJ,Fallon M,et al.Contralateral sound suppresses distortion product otoacoustic emissions through cholinergic mechanisms[J].Hear Res,1993,68:97.

18Atcherson SR,Martin MJ,Lintvedt R.Contralateral noise has possible asymmetric frequency-sensitive effect on2F1-F2otoacoustic emission in humans[J].Neuroscience Letters,2008,438:107.

19Wittekindt A,Gaese BH,Kossl M.Influence of contralateral acoustic sti mulation onthe quadratic distortion product f2-f1in humans[J].Hearing Research,2009,247:27.

20John J,Guinan Jr.Olivocochlear efferents:Anatomy,physi-ology,function,and the measurement of efferent effects in humans[J].Ear&Hearing,2006,27:589.

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