胎鼠神经干细胞的原代培养及体外诱导分化研究
Primary Culture of Neurospheres Obtained from Fetal Mouse Central Nervous System and Differentiation of Inner Ear Hair Cell Immunophenotypes in Vitro
刘晓薇;孙敬武;
1:安徽省立医院耳鼻咽喉科
摘要关键词基金项目(Foundation):作者参考文献1Bjornson CR,Rietze RL,Reynolds BA,et al.Turning brain into blood:Ahematopoietic fate adopted by adult neural stem cells in vivo[see comments][J].Science,1999,283:534.
2Weir J,Rivolta M,Holley M.Identification of differentiating cochlear hair cells in vitro[J].AmJ Otol,2000,21:130.
3Lawlor P,Marcotti W,Rivolta MN,et al.Differentiation of mammalian vestibular hair cells from conditionally i mmortal,postnatal supporting cells[J].J Neurosci,1999,19:9445.
4Malgrange B,Belachew S,Thiry M,et al.Prolifrative genera-tion of mammalian auditory hair cells in culture[J].Mech Dev,2002,112:79.
5Zhao HB.Long-termnatural culture of cochlear sensory epi-thelia of guinea pigs[J].Neurosci Lett,2001,315:73.
6Frenz DA,Vandewater TR.Transforminggro wthfactor-be-ta:Does it direct otic capsule formation[J].Ann Otol Rhinol Laryngol,1991,100:301.
7Frenz DA,Galinovic-Schwartz V,Liu W,et al.Transforming growth factor B1is an epithelial-deried signal peptide that in-fluences otic capsule formations[J].Dev Biol,1992,153:324.
8Weiss S,Reynolds BA,Vescovi AL,et al.Isthere a neural stemcell in the mammalianforebrain[J]-TINS,1996,19:387.
9Reynolds BW,Weiss S.Clonal and population analyses demon-strate that an EGF-responsive mammalian embryonic CNS precursor is a stemcell[J].Dev Biol,1996,175:1.
10Reynolds BA,Weiss S.Generation of neurons and astrocytes fromisolated cells of the adult mammalian central nervous system[J].Science,1992,255:1707.
11Carpenter MK,Cui X,Hu ZY,et al.In vitro expansion of a multipotent population of human neural progenitor cells[J].Exp Neurol,1999,158:265.
12Shihabuddin LS,Ray J,Gage FH.FGF-2is sufficient to isolate progenitors found in the adult mammalian spinal cord[J].Exp Neurol,1997,148:577.
13Johe KK,Hazel TG,Muller T,et al.Single factors direct the differentiation of stemcells fromthe fetal and adult cen-tral nervous system[J].Gene Dev,1996,10:3129.
14Whittemore SR,Morassutti DJ,Walters WM,et al.Mito-gen and substrate differentially affect thelineage restriction of adult rat subventricular zone neural precursor cell populations[J].Exp Cell Res,1999,252:75.
15Lawlor P,Marcotti W,Rivolta MN,et al.Differentiation of mammalian vestibular hair cells fromconditionally i mmortal,postnatal supporting cells[J].J Neurosci,1999,19:9445.
16Malgrange B,Belachew S,Thiry M,et al.Proliferative ge-neration of mammalian auditory hair cellsin culture[J].Mech Dev,2002,112:79.
17Koji ma K,Tamura S,Nishida AT,et al.Generation of inner ear hair cell i mmunophenotypes from neurospheres obtained fromfetal rat central nervous systemin vitro[J].Acta Oto-laryngol,2004(Suppl),551:26.
18Johansson CB,Lothian C,Molin M,et al.Nestin enhancerrequirements for expressionin normal and injured adult CNS[J].J Neurosci Res,2002,69:784.
19Mignone JL,Kukekov V,Chiang AS,et al.Neural stemand progenitor cells in nestin-GFP transgenic mice[J].J Comp Neurol,2004,469:311.
20Yamaguchi M,Saito H,Suzuki M,et al.Visualization of neurogenesis in the central nervous system using nestin pro-moter-GFP transgenic mice[J].Neuroreport,2000,11:1991.
21Walshe P,Walsh M,McConn Wash R.Hair cell regeneration in the inner ear:Areview[J].Clin Otolaryngol,2003,28:5.
22Li H,Liu H,Heller S.Pluripotent stemcells fromthe adult mouse inner ear[J].Nat Med,2003,9:1293.
23赵玉林,李晓萍,董明敏,等.转化生长因子-β1在小鼠内耳发育中作用的研究[J].中国民政医学杂志,2002,14:72.