β-防御素在声带息肉中的表达及意义

The Tissue Microarray Technology and Its Preliminary Application in Evaluation of the Expression and Clinical Significance of hBD in the Vocal Cord Polyp

谷津津;黄永旺;

1:天津医科大学第二医院耳鼻咽喉科

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摘要

目的探讨人类β-防御素1(human beta-defensin1,hBD-1)、人类β-防御素2(human beta-defensin2,hBD-2)在声带息肉组织中的表达及意义。方法采用组织芯片技术制作包括声带息肉组、声带小结组以及对照组(正常声带组织)标本的组织芯片,利用S-P免疫组织化学染色法检测hBD-1、hBD-2在各组的表达情况。结果hBD-1在声带息肉、声带小结及对照组中均为低强度表达,且三组间差异无统计学意义(P>0.05);hBD-2在声带小结组和对照组中仅有微弱表达,而在声带息肉组中表达明显增强(P<0.05)。结论hBD-1和hBD-2在声带上皮中分别为固有性表达和诱导性表达,这表明声带上皮在机体细胞和体液免疫尚未发生作用之前,即可通过分泌β-防御素及时、有效地杀灭外来病原菌,在早期炎症反应中发挥天然防御作用。

关键词

声带息肉;β-防御素-1;β-防御素-2;免疫组化染色;组织芯片

基金项目(Foundation):

作者

谷津津;黄永旺;

参考文献

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声带息肉 β-防御素-1 β-防御素-2 免疫组化染色组织芯片

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谷津津黄永旺