人凝血因子C同源物基因全长cDNA克隆

Full Length cDNA Cloning of Human Coagulation Factor C Homology

孙勍;程静;王国建;刘新;于飞;韩冰;陈静;戴朴;袁慧军;韩东一;

1:武警总医院耳鼻咽喉头颈外科

2:解放军总医院耳鼻咽喉科研究所

摘要
目的通过克隆凝血因子C同源物基因(Coagulation factor C homology,COCH)全长cDNA,为COCH编码蛋白cochlin的功能研究打下基础。方法从听力正常人新鲜外周静脉血中提取总RNA,应用一步法RT-PCR试剂盒进行COCH反转录,转录产物进行浓缩、酶切、连接。结果反转录COCH cDNA全长与标准基因序列比较,结果完全相符,得到完整的COCH cDNA全长序列。结论本研究成功克隆了COCH cDNA全长序列,为COCH编码蛋白cochlin的功能研究打下了良好的基础。
关键词
克隆;凝血因子C同源物基因;互补DNA
基金项目(Foundation):
国家自然科学基金面上项目(30371523);; 国家自然科学基金面上项目(30571018);; 国家自然科学基金海外青年学者合作基金(30528025)资助
作者
孙勍;程静;王国建;刘新;于飞;韩冰;陈静;戴朴;袁慧军;韩东一;
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