水杨酸钠对大鼠耳蜗NKCC_1 mRNA表达的影响

The Influence of Sodium Salicylate on the Expression of Na~+-K~+-2Cl~-

姚可青;黄治物;杨琨;黄娟;

1:武汉大学人民医院耳鼻咽喉-头颈外科

摘要

目的研究水杨酸钠作用后大鼠耳蜗Na+-K+-2Cl-联合转运体(Na+-K+-2Cl-co-trans-porter,NKCC1)mRNA的表达变化情况,探讨NKCC1在水杨酸钠肌注后引起大鼠耳蜗主动性改变中的作用和意义。方法选用24只正常成年SD大鼠,随机分成四组,每组6只:正常对照组(无特殊处理)、急性组(一次肌注水杨酸钠400mg/kg后处死)、慢性组(肌注水杨酸钠175mg/kg,2次/天,连续用药l4天后处死),恢复组(给药方法及时间同慢性组,停药l4天后处死),运用荧光定量PCR技术比较四组大鼠耳蜗NKCC1 mRNA的表达变化。结果NKCC1 mRNA在正常对照组、急性组、慢性组、恢复组均有表达,慢性组、恢复组的表达量均高于正常组,差异均有统计学意义(P<0.05);急性组NKCC1 mRNA的表达低于正常组,差异有统计学意义(P<0.05);恢复组NKCC1 mRNA的表达低于慢性组,差异有统计学意义(P<0.05)。结论NKCC1 mRNA在正常大鼠中有表达,提示其对维持内淋巴液中Cl-的平衡有一定作用;水杨酸钠肌注可以引起大鼠耳蜗NKCC1 mRNA表达变化,可能通过改变耳蜗内淋巴液Cl-浓度平衡,而影响外毛细胞的电能动性。

关键词

NKCC1;水杨酸钠;外毛细胞电能动性;荧光定量PCR

基金项目(Foundation):

作者

姚可青;黄治物;杨琨;黄娟;

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NKCC1 水杨酸钠外毛细胞电能动性荧光定量PCR

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姚可青黄治物杨琨黄娟