黄芪对顺铂耳毒性保护作用的实验研究

The Protective Role of Radix Astragali against Ototoxicity Caused by Cisplatin

田颖;于刚;姜学钧;

1:中国医科大学附属第一医院耳鼻咽喉科

摘要
目的探讨黄芪是否对顺铂的耳毒性具有保护作用。方法健康SD大鼠40只随机分成4组,每组10只。对照组:生理盐水2ml/d腹腔注射6天;黄芪组:黄芪注射液5g·kg-1·d-1腹腔注射6天;顺铂组:顺铂4mg·kg-1·d-1腹腔注射6天;顺铂加黄芪组:腹腔注射黄芪5g·kg-1.d-1加顺铂4mg·kg-1·d-16天。用药前及用药后第7天行畸变产物耳声发射(DPOAE)检测,然后处死大鼠。每组半数动物冰冻连续切片,DNA末端转移酶介导的缺口末端标记法(TUNEL)检测毛细胞凋亡;半数动物扫描电镜观察毛细胞形态。结果顺铂组用药后DPOAE幅值下降,毛细胞受损,并可观察到凋亡细胞,与对照组及黄芪组比较差异具有显著统计学意义(P<0.01)。顺铂加黄芪组用药后DPOAE幅值上升,毛细胞受损减轻,凋亡细胞数量减少,与顺铂组比较,差异有统计学意义(P<0.05)。结论黄芪能有效保护耳蜗免受顺铂的耳毒性损伤。
关键词
顺铂;黄芪;耳毒性;大鼠;毛细胞
基金项目(Foundation):
作者
田颖;于刚;姜学钧;
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