斯里兰卡肉桂碱受体在不同鼠龄大鼠耳蜗中的表达差异

Differential Expression of Ryanodine Receptors in the Developing Rat Cochlea

梁玉芳;杨军;

1:上海交通大学医学院附属新华医院耳鼻咽喉-头颈外科

摘要
目的研究不同鼠龄大鼠耳蜗内斯里兰卡肉桂碱受体(ryanodine receptor,RyR)的差异表达,探讨RyR表达与耳蜗发育成熟的关系。方法分别选取出生后1天(P1)、5天(P5)、10天(P10)、14天(P14)、28天(P28)以及成年SD大鼠(>2月龄)各5只耳蜗作冰冻切片,运用免疫荧光的方法,比较各组大鼠耳蜗组织RyR的表达差异。结果P1组和P5组大鼠耳蜗Corti器内RyR表达不明显,P10组出现的RyR染色均匀,而P14组大鼠耳蜗Corti器内RyR的表达出现明显特征性变化,毛细胞突触区RyR强表达,而支持细胞内的表达相对较为广泛。大鼠出生后耳蜗螺旋神经元内RyR的表达由广泛的胞内表达,逐渐趋近细胞膜突触区。结论RyR在大鼠出生后14天表达基本成熟,与耳蜗的发育成熟基本保持一致。感觉毛细胞和螺旋神经元的RyR表达可能与神经传递等功能相关。在发育早期的螺旋神经元细胞中RyR的广泛表达,可能参与了螺旋神经元发育成熟中的细胞凋亡过程。
关键词
斯里兰卡肉桂碱受体;耳蜗发育;受体表达;钙诱导钙释放;钙
基金项目(Foundation):
国家自然科学基金资助(30772396)
作者
梁玉芳;杨军;
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